50525

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 36381..37006 Replicon plasmid B
Accession NZ_CP010124
Organism Escherichia coli strain C5

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L4J644
Locus tag RG28_RS29860 Protein ID WP_061358607.1
Coordinates 36381..36779 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L4J6D3
Locus tag RG28_RS29865 Protein ID WP_073521399.1
Coordinates 36779..37006 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG28_RS29840 33574..34224 + 651 WP_073521416.1 hypothetical protein -
RG28_RS29845 34296..35033 + 738 WP_073521392.1 complement resistance protein TraT -
RG28_RS29850 35162..35854 + 693 WP_073521394.1 hypothetical protein -
RG28_RS29855 35861..36364 + 504 WP_073521396.1 DUF2726 domain-containing protein -
RG28_RS29860 36381..36779 - 399 WP_061358607.1 tRNA(fMet)-specific endonuclease VapC Toxin
RG28_RS29865 36779..37006 - 228 WP_073521399.1 toxin-antitoxin system antitoxin VapB Antitoxin
RG28_RS29870 37139..39328 + 2190 WP_061361235.1 type IV conjugative transfer system coupling protein TraD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..86032 86032


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14945.31 Da        Isoelectric Point: 8.1432

>T50525 WP_061358607.1 NZ_CP010124:c36779-36381 [Escherichia coli]
MLKFMLDTNICIFTIKNKPAHIRERFNLNTGRMCISSVTVMELVYGAEKSQFPERNLAIIEGFISRLDVLDYDCAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTSNTREFERIAGLRLEDWC

Download         Length: 399 bp

>T50525 NZ_CP010124:c36779-36381 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACCATCAAAAATAAACCAGCGCATATCCGGGAGCGTTTCAA
TCTGAATACAGGGCGAATGTGCATCAGCTCAGTGACCGTGATGGAACTGGTATACGGTGCTGAAAAAAGTCAGTTTCCGG
AGCGTAATCTCGCGATTATCGAAGGATTTATTTCGCGCCTTGATGTGCTGGATTACGACTGCGCTGCTGCCACACATACC
GGTCAGATCCGTGCTGAGCTCGCCCGTCAGGGACGTCCGGTTGGGCCGTTTGATCAGATGATTGCCGGTCATGCCCGCAG
CCGGGGACTCATTGTGGTCACAAGCAATACCCGGGAGTTTGAACGTATCGCCGGCCTTCGTCTGGAAGACTGGTGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8354.33 Da        Isoelectric Point: 4.3569

>AT50525 WP_073521399.1 NZ_CP010124:c37006-36779 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVDVIAVGRTRIITPAGESWDSWFDGDGVSSDFMNDREQPAVQERDAF

Download         Length: 228 bp

>AT50525 NZ_CP010124:c37006-36779 [Escherichia coli]
ATGGAAACCACAGTATTTCTCAGTAACCGGAGTCAGGCAGTCAGGCTGCCGAAAGCTGTAGCCCTGCCTGAAGACGTAAA
AAAAGTCGACGTTATTGCTGTTGGCCGTACCCGGATCATTACCCCGGCAGGAGAGTCCTGGGACAGCTGGTTCGATGGCG
ACGGTGTCAGCTCAGATTTCATGAATGACAGGGAACAACCCGCCGTACAGGAAAGGGACGCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L4J644

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4J6D3

References