Detailed information of TA system TA050520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 191301..192636
• Replicon: plasmid A
• Accession: NZ_CP010123
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: RG28_RS29460
• Protein ID: WP_057697651.1
• Coordinates: 191689..192636 (+)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A0Q1C5A3
• Locus tag: RG28_RS29455
• Protein ID: WP_000208385.1
• Coordinates: 191301..191696 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS29410	186533..187351	+	819	WP_073521340.1	DUF945 domain-containing protein	-
RG28_RS29415	187443..187928	+	486	WP_073521341.1	antirestriction protein	-
RG28_RS29420	187944..188420	+	477	WP_073521342.1	RadC family protein	-
RG28_RS29425	188477..188698	+	222	WP_063073973.1	DUF987 domain-containing protein	-
RG28_RS29430	188773..189141	+	369	WP_073521343.1	type IV toxin-antitoxin system YeeU family antitoxin	-
RG28_RS29435	189231..189608	+	378	WP_073521344.1	type IV toxin-antitoxin system toxin CbtA	-
RG28_RS29440	189605..190093	+	489	WP_073521345.1	hypothetical protein	-
RG28_RS29445	190105..190302	+	198	WP_073521346.1	DUF957 domain-containing protein	-
RG28_RS29450	190387..191232	+	846	WP_073521347.1	DUF4942 domain-containing protein	-
RG28_RS29455	191301..191696	+	396	WP_000208385.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
RG28_RS29460	191689..192636	+	948	WP_057697651.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
RG28_RS29465	193040..194305	+	1266	WP_001218789.1	integrase arm-type DNA-binding domain-containing protein	-
RG28_RS29470	194624..196123	-	1500	WP_069904771.1	phosphoethanolamine transferase	-
RG28_RS29475	196275..196982	-	708	WP_073521348.1	porin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pefD / pefC / pefA / papD / papC	1..214445	214445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-302)

Antitoxin

• Protein of unknown function DUF6088 (10-129)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 36240.66 Da
• Isoelectric Point: 6.3550

>T50520 WP_057697651.1 NZ_CP010123:191689-192636 [Escherichia coli]
MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETHTLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKKIVQIITDVINNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMESTLLEHPESR
DIYSLVTELTGKGTPVTAFPCATILSTQAEKLISMMRRTAAHLRNPEQQDDEFLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQYPQFCSSPVDELKKGLDALRDDPVHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTS

Nucleotide

• Length: 948 bp

>T50520 NZ_CP010123:191689-192636 [Escherichia coli]
ATGCCTGAATTAACATCATTATTTCCTGACGTTGCCAACGCACTGGATATTGAATCTGTCGCTATCGTCGAAAAAGATTA
TTTTGTGGTTAATCTTTTAAAGTTACTAAAAGAAATAAAACCAGAAACACATACTCTCGTGTTTGCAGGAGGTACCGCAT
TGTCAAAAGCAGGTATCTCATTGAACAGAATGTCAGAAGATATTGATATAAAGCTCGTGCCAACAGAGAACTTTATGCAA
AATGGCAGAGACAAGAGAAGAAAAATTCGCAAGAAAATCGTGCAAATAATCACGGATGTTATTAATAATTCTGACATTTT
CAGCCTGGATAATGAAAACGCCCGTATAACACGTGATGAGTATCGTTATAACGAAATCTCGGTACGTTATCCCCAGACAT
TTGCGCAAGTCCCCTGTCTTCGTCCATTTATTAAGCTGGAGTTGATGGAAAGCACCCTGCTGGAACATCCGGAAAGCAGA
GATATTTATTCACTTGTCACGGAGTTAACGGGTAAAGGTACACCTGTAACAGCATTCCCCTGTGCCACCATTTTGTCCAC
TCAGGCTGAAAAGCTCATCTCCATGATGCGAAGAACAGCAGCACATCTTCGTAATCCCGAACAACAGGATGATGAGTTCC
TGGTACGTCACATTTACGACAACTACTGCATTGTCAGGGAAAAAGGTGTGAATGTTCCTGTATTGAAAAATTTTGTTCAG
ATATGTATTCAACAGGATATTGAACGCTATGGTAACCAGTACCCACAATTTTGTAGCTCACCAGTTGATGAACTTAAGAA
AGGGCTGGATGCACTCCGGGATGATCCTGTACACAAATTGCGTTTTCAACGGTTTGTTAAACCGATGGTATTTGGTAATA
CACAGATTGACTGGGAAGAAGCCTACTCCTGTTTCAGACAAACAGCATTGAGTGTTCTGACCAGCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14595.97 Da
• Isoelectric Point: 10.9440

>AT50520 WP_000208385.1 NZ_CP010123:191301-191696 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITE
ALDLLNIRYSLIGATECYNKGQSTQIPVSPEFKTPPRFKRVLVVGKSKINA

Nucleotide

• Length: 396 bp

>AT50520 NZ_CP010123:191301-191696 [Escherichia coli]
ATGACTACAAAAGACCGTATTCAGTCCCGACTGAACCGTTCAAAGCGTTATGTGTTCACCCGGGATGACTTCAGGGATAT
CGCTGGCTATGATCAGGTTGGCAGAGCTCTTCGTACACTGGTAAACGAAGGTAAGTTGATGAAGGTGGGCTATGGTGTTT
ACACCAAAGCCCGTCGCAACGTTATTACAGGAAAAATAATGCCTGCAGCACCTGGTGGCTCTGCTGCTGTAATCACCGAA
GCCCTGGATCTTTTGAATATCAGATACAGCTTGATCGGCGCAACAGAATGCTACAATAAAGGTCAGTCCACACAGATTCC
GGTGTCTCCGGAGTTCAAAACACCTCCTCGCTTTAAAAGAGTGTTAGTTGTAGGGAAAAGTAAAATCAATGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0Q1C5A3