Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 188773..189608 Replicon plasmid A
Accession NZ_CP010123
Organism Escherichia coli strain C5

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1L4J657
Locus tag RG28_RS29435 Protein ID WP_073521344.1
Coordinates 189231..189608 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1L4J5W8
Locus tag RG28_RS29430 Protein ID WP_073521343.1
Coordinates 188773..189141 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG28_RS29395 184593..185477 + 885 WP_073521338.1 50S ribosome-binding GTPase -
RG28_RS29400 185598..186275 + 678 WP_073521339.1 hypothetical protein -
RG28_RS29410 186533..187351 + 819 WP_073521340.1 DUF945 domain-containing protein -
RG28_RS29415 187443..187928 + 486 WP_073521341.1 antirestriction protein -
RG28_RS29420 187944..188420 + 477 WP_073521342.1 RadC family protein -
RG28_RS29425 188477..188698 + 222 WP_063073973.1 DUF987 domain-containing protein -
RG28_RS29430 188773..189141 + 369 WP_073521343.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
RG28_RS29435 189231..189608 + 378 WP_073521344.1 type IV toxin-antitoxin system toxin CbtA Toxin
RG28_RS29440 189605..190093 + 489 WP_073521345.1 hypothetical protein -
RG28_RS29445 190105..190302 + 198 WP_073521346.1 DUF957 domain-containing protein -
RG28_RS29450 190387..191232 + 846 WP_073521347.1 DUF4942 domain-containing protein -
RG28_RS29455 191301..191696 + 396 WP_000208385.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein -
RG28_RS29460 191689..192636 + 948 WP_057697651.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
RG28_RS29465 193040..194305 + 1266 WP_001218789.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pefD / pefC / pefA / papD / papC 1..214445 214445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14045.09 Da        Isoelectric Point: 7.9086

>T50519 WP_073521344.1 NZ_CP010123:189231-189608 [Escherichia coli]
MKTLTVLPGQAASFRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCNAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Download         Length: 378 bp

>T50519 NZ_CP010123:189231-189608 [Escherichia coli]
ATGAAAACATTAACTGTATTACCCGGGCAGGCGGCCAGTTTTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGTCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCAATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13675.40 Da        Isoelectric Point: 6.3146

>AT50519 WP_073521343.1 NZ_CP010123:188773-189141 [Escherichia coli]
VSDTLHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSKTKK

Download         Length: 369 bp

>AT50519 NZ_CP010123:188773-189141 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCAAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L4J657

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4J5W8

References