Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 188773..189608 | Replicon | plasmid A |
| Accession | NZ_CP010123 | ||
| Organism | Escherichia coli strain C5 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A1L4J657 |
| Locus tag | RG28_RS29435 | Protein ID | WP_073521344.1 |
| Coordinates | 189231..189608 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A1L4J5W8 |
| Locus tag | RG28_RS29430 | Protein ID | WP_073521343.1 |
| Coordinates | 188773..189141 (+) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RG28_RS29395 | 184593..185477 | + | 885 | WP_073521338.1 | 50S ribosome-binding GTPase | - |
| RG28_RS29400 | 185598..186275 | + | 678 | WP_073521339.1 | hypothetical protein | - |
| RG28_RS29410 | 186533..187351 | + | 819 | WP_073521340.1 | DUF945 domain-containing protein | - |
| RG28_RS29415 | 187443..187928 | + | 486 | WP_073521341.1 | antirestriction protein | - |
| RG28_RS29420 | 187944..188420 | + | 477 | WP_073521342.1 | RadC family protein | - |
| RG28_RS29425 | 188477..188698 | + | 222 | WP_063073973.1 | DUF987 domain-containing protein | - |
| RG28_RS29430 | 188773..189141 | + | 369 | WP_073521343.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| RG28_RS29435 | 189231..189608 | + | 378 | WP_073521344.1 | type IV toxin-antitoxin system toxin CbtA | Toxin |
| RG28_RS29440 | 189605..190093 | + | 489 | WP_073521345.1 | hypothetical protein | - |
| RG28_RS29445 | 190105..190302 | + | 198 | WP_073521346.1 | DUF957 domain-containing protein | - |
| RG28_RS29450 | 190387..191232 | + | 846 | WP_073521347.1 | DUF4942 domain-containing protein | - |
| RG28_RS29455 | 191301..191696 | + | 396 | WP_000208385.1 | type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein | - |
| RG28_RS29460 | 191689..192636 | + | 948 | WP_057697651.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
| RG28_RS29465 | 193040..194305 | + | 1266 | WP_001218789.1 | integrase arm-type DNA-binding domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | pefD / pefC / pefA / papD / papC | 1..214445 | 214445 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14045.09 Da Isoelectric Point: 7.9086
>T50519 WP_073521344.1 NZ_CP010123:189231-189608 [Escherichia coli]
MKTLTVLPGQAASFRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCNAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ
MKTLTVLPGQAASFRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCNAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ
Download Length: 378 bp
>T50519 NZ_CP010123:189231-189608 [Escherichia coli]
ATGAAAACATTAACTGTATTACCCGGGCAGGCGGCCAGTTTTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGTCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCAATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA
ATGAAAACATTAACTGTATTACCCGGGCAGGCGGCCAGTTTTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGTCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCAATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAACAATGA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13675.40 Da Isoelectric Point: 6.3146
>AT50519 WP_073521343.1 NZ_CP010123:188773-189141 [Escherichia coli]
VSDTLHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSKTKK
VSDTLHETNYPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTSKTKK
Download Length: 369 bp
>AT50519 NZ_CP010123:188773-189141 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCAAAACGAAAAAGTAA
GTGTCAGACACACTCCATGAGACAAATTATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCAAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1L4J657 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1L4J5W8 |