Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 96075..96718 Replicon plasmid A
Accession NZ_CP010123
Organism Escherichia coli strain C5

Toxin (Protein)


Gene name vagD Uniprot ID A0A1L4J633
Locus tag RG28_RS28915 Protein ID WP_047612429.1
Coordinates 96302..96718 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A627FA37
Locus tag RG28_RS28910 Protein ID WP_001604445.1
Coordinates 96075..96305 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG28_RS28895 94632..95126 - 495 WP_024195926.1 type II toxin-antitoxin system YhaV family toxin -
RG28_RS28900 95149..95493 - 345 WP_001559862.1 type II toxin-antitoxin system PrlF family antitoxin -
RG28_RS28905 95737..95814 - 78 Protein_116 helix-turn-helix domain-containing protein -
RG28_RS28910 96075..96305 + 231 WP_001604445.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
RG28_RS28915 96302..96718 + 417 WP_047612429.1 type II toxin-antitoxin system VapC family toxin Toxin
RG28_RS28920 96858..97838 + 981 WP_073521305.1 hypothetical protein -
RG28_RS28925 98050..99621 - 1572 WP_047613524.1 ATP-binding protein -
RG28_RS28930 99940..100209 + 270 WP_073521306.1 hypothetical protein -
RG28_RS28935 100197..100793 + 597 WP_059219358.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - pefD / pefC / pefA / papD / papC 1..214445 214445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15064.41 Da        Isoelectric Point: 6.7125

>T50518 WP_047612429.1 NZ_CP010123:96302-96718 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLILEDWAG

Download         Length: 417 bp

>T50518 NZ_CP010123:96302-96718 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTTCAGCTGGTTGACGCATTCTGTGAGCGACTCGATGCCGTCCTGCCCTGGGATCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTTCCGGGCCTGATACTGG
AAGACTGGGCCGGTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8736.84 Da        Isoelectric Point: 4.4692

>AT50518 WP_001604445.1 NZ_CP010123:96075-96305 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVISDEGRFNL

Download         Length: 231 bp

>AT50518 NZ_CP010123:96075-96305 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGTGCCATCCGTCTGCCCCGGGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTATCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L4J633

Antitoxin

Source ID Structure
AlphaFold DB A0A627FA37

References