Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/DUF1778(antitoxin) |
| Location | 25973..26748 | Replicon | plasmid A |
| Accession | NZ_CP010123 | ||
| Organism | Escherichia coli strain C5 | ||
Toxin (Protein)
| Gene name | tacT | Uniprot ID | A0A0C2EI97 |
| Locus tag | RG28_RS28420 | Protein ID | WP_000405250.1 |
| Coordinates | 25973..26455 (-) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | tacA | Uniprot ID | - |
| Locus tag | RG28_RS28425 | Protein ID | WP_072146426.1 |
| Coordinates | 26446..26748 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RG28_RS28375 | 21284..21685 | - | 402 | WP_149027148.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| RG28_RS31565 | 21618..21875 | - | 258 | WP_000557618.1 | type II toxin-antitoxin system antitoxin PemI | - |
| RG28_RS28385 | 21968..22621 | - | 654 | WP_040100282.1 | CPBP family intramembrane metalloprotease | - |
| RG28_RS28395 | 23562..24584 | - | 1023 | WP_042973996.1 | replication initiation protein | - |
| RG28_RS28400 | 24574..25107 | - | 534 | WP_040089446.1 | replication protein | - |
| RG28_RS28405 | 25100..25174 | - | 75 | WP_001367777.1 | RepA leader peptide Tap | - |
| RG28_RS28415 | 25408..25665 | - | 258 | WP_001136637.1 | replication regulatory protein RepA | - |
| RG28_RS28420 | 25973..26455 | - | 483 | WP_000405250.1 | GNAT family N-acetyltransferase | Toxin |
| RG28_RS28425 | 26446..26748 | - | 303 | WP_072146426.1 | DUF1778 domain-containing protein | Antitoxin |
| RG28_RS28430 | 27069..27275 | - | 207 | WP_040089444.1 | type I toxin-antitoxin system Hok family toxin | - |
| - | 27262..27323 | + | 62 | NuclAT_1 | - | - |
| - | 27262..27323 | + | 62 | NuclAT_1 | - | - |
| - | 27262..27323 | + | 62 | NuclAT_1 | - | - |
| - | 27262..27323 | + | 62 | NuclAT_1 | - | - |
| RG28_RS28440 | 27564..27773 | - | 210 | WP_073521270.1 | hemolysin expression modulator Hha | - |
| RG28_RS28445 | 27830..28066 | - | 237 | WP_061358569.1 | hypothetical protein | - |
| RG28_RS28450 | 28313..28873 | - | 561 | WP_073521271.1 | fertility inhibition protein FinO | - |
| RG28_RS28455 | 28928..29671 | - | 744 | WP_073521272.1 | conjugal transfer pilus acetylase TraX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | pefD / pefC / pefA / papD / papC | 1..214445 | 214445 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17649.42 Da Isoelectric Point: 7.0386
>T50509 WP_000405250.1 NZ_CP010123:c26455-25973 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEGTLRVVGFYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNVAEQVGVKAVMVHAIDDDARAFYERFGFVQSVVAPDTLFYKV
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEGTLRVVGFYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNVAEQVGVKAVMVHAIDDDARAFYERFGFVQSVVAPDTLFYKV
Download Length: 483 bp
>T50509 NZ_CP010123:c26455-25973 [Escherichia coli]
ATGGAAATAAATGTCACCGCGCCGGCATTGCTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTTATTTGCCTGGAGGGGA
CTTTACGTGTTGTGGGGTTCTACTCATTAGCTACGGGTTCCGTCACACATGCCGAGCTTGGGCGCAGTTTGCGGCATAAC
ATGCCTAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTGCTGAGTGATGCTATTCACCGGGTTGTAAATGTGGCTGAACAGGTTGGCGTTAAGGCGGTTATGGTGCATGCAATTG
ATGATGACGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCTGACACCTTATTTTATAAAGTC
TGA
ATGGAAATAAATGTCACCGCGCCGGCATTGCTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTTATTTGCCTGGAGGGGA
CTTTACGTGTTGTGGGGTTCTACTCATTAGCTACGGGTTCCGTCACACATGCCGAGCTTGGGCGCAGTTTGCGGCATAAC
ATGCCTAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTGCTGAGTGATGCTATTCACCGGGTTGTAAATGTGGCTGAACAGGTTGGCGTTAAGGCGGTTATGGTGCATGCAATTG
ATGATGACGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCTGACACCTTATTTTATAAAGTC
TGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11648.21 Da Isoelectric Point: 4.7174
>AT50509 WP_072146426.1 NZ_CP010123:c26748-26446 [Escherichia coli]
MEVFMSTAASVRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK
MEVFMSTAASVRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK
Download Length: 303 bp
>AT50509 NZ_CP010123:c26748-26446 [Escherichia coli]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAAAACCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAACTGGCTTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGCTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAAAACCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAACTGGCTTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGCTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|