50504

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4423932..4424563 Replicon chromosome
Accession NZ_CP010122
Organism Escherichia coli strain C5

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag RG28_RS23595 Protein ID WP_001259385.1
Coordinates 4424288..4424563 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag RG28_RS23590 Protein ID WP_000593555.1
Coordinates 4423932..4424291 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG28_RS23560 4419044..4419517 + 474 WP_001161650.1 PTS sugar transporter subunit IIA -
RG28_RS23565 4419514..4419795 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
RG28_RS23570 4419872..4421230 + 1359 WP_021565455.1 PTS galactitol transporter subunit IIC -
RG28_RS23575 4421223..4422731 + 1509 WP_021565454.1 xylulose kinase -
RG28_RS23580 4422721..4422990 + 270 WP_000933061.1 HPr family phosphocarrier protein -
RG28_RS23585 4423022..4423882 + 861 WP_001131788.1 class II aldolase -
RG28_RS23590 4423932..4424291 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
RG28_RS23595 4424288..4424563 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
RG28_RS23600 4424622..4425746 - 1125 WP_021565453.1 ABC transporter permease -
RG28_RS23605 4425746..4428481 - 2736 WP_073521150.1 ribosome-associated ATPase/putative transporter RbbA -
RG28_RS23610 4428478..4429545 - 1068 WP_021565451.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4418273..4433384 15111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T50504 WP_001259385.1 NZ_CP010122:c4424563-4424288 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T50504 NZ_CP010122:c4424563-4424288 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT50504 WP_000593555.1 NZ_CP010122:c4424291-4423932 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT50504 NZ_CP010122:c4424291-4423932 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References