Detailed information of TA system TA050502

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3973846..3974644
• Replicon: chromosome
• Accession: NZ_CP010122
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A162PM82
• Locus tag: RG28_RS21210
• Protein ID: WP_063848187.1
• Coordinates: 3973846..3974223 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A167CM00
• Locus tag: RG28_RS21215
• Protein ID: WP_063848188.1
• Coordinates: 3974270..3974644 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS21180	3969476..3971317	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
RG28_RS21185	3971396..3971902	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
RG28_RS21195	3972202..3973047	-	846	WP_063848184.1	DUF4942 domain-containing protein	-
RG28_RS21200	3973144..3973341	-	198	WP_063848185.1	DUF957 domain-containing protein	-
RG28_RS21205	3973361..3973849	-	489	WP_063848186.1	hypothetical protein	-
RG28_RS21210	3973846..3974223	-	378	WP_063848187.1	TA system toxin CbtA family protein	Toxin
RG28_RS21215	3974270..3974644	-	375	WP_063848188.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
RG28_RS21220	3974694..3975338	-	645	WP_063848189.1	hypothetical protein	-
RG28_RS21225	3975353..3975574	-	222	WP_063848190.1	DUF987 domain-containing protein	-
RG28_RS21230	3975643..3976119	-	477	WP_073521125.1	RadC family protein	-
RG28_RS21235	3976135..3976620	-	486	WP_057697935.1	antirestriction protein	-
RG28_RS21240	3976712..3977530	-	819	WP_073521126.1	DUF945 domain-containing protein	-
RG28_RS21255	3977823..3978278	-	456	WP_005051844.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14174.18 Da
• Isoelectric Point: 7.3524

>T50502 WP_063848187.1 NZ_CP010122:c3974223-3973846 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T50502 NZ_CP010122:c3974223-3973846 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAATTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13651.38 Da
• Isoelectric Point: 6.6240

>AT50502 WP_063848188.1 NZ_CP010122:c3974644-3974270 [Escherichia coli]
VSDTLSGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT50502 NZ_CP010122:c3974644-3974270 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCGCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGACACCCTCGGTAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACACCGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A162PM82

Antitoxin

• AlphaFold DB: A0A167CM00