Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3845046..3845881 Replicon chromosome
Accession NZ_CP010122
Organism Escherichia coli strain C5

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1L4J186
Locus tag RG28_RS20500 Protein ID WP_000854742.1
Coordinates 3845504..3845881 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1L4J164
Locus tag RG28_RS20495 Protein ID WP_001406130.1
Coordinates 3845046..3845414 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG28_RS20470 3842707..3843525 + 819 WP_001234392.1 DUF945 domain-containing protein -
RG28_RS20475 3843617..3844102 + 486 WP_000213706.1 antirestriction protein -
RG28_RS20480 3844117..3844593 + 477 WP_021566731.1 RadC family protein -
RG28_RS20485 3844662..3844883 + 222 WP_000692345.1 DUF987 domain-containing protein -
RG28_RS20495 3845046..3845414 + 369 WP_001406130.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
RG28_RS20500 3845504..3845881 + 378 WP_000854742.1 TA system toxin CbtA family protein Toxin
RG28_RS20505 3845878..3846366 + 489 WP_000777551.1 hypothetical protein -
RG28_RS20510 3846378..3846575 + 198 WP_000839267.1 DUF957 domain-containing protein -
RG28_RS31420 3847771..3848280 + 510 WP_187655784.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC 3788869..3893303 104434


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14236.28 Da        Isoelectric Point: 8.2976

>T50501 WP_000854742.1 NZ_CP010122:3845504-3845881 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTHSQLINSIDILRARRATGLMTRSNYRMVNDITRGKHSEAKR

Download         Length: 378 bp

>T50501 NZ_CP010122:3845504-3845881 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCATTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACCCACTCTCAGTTAATAAACAGCATCGATATTCTACGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCACCCGGGGTAAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13717.53 Da        Isoelectric Point: 7.0269

>AT50501 WP_001406130.1 NZ_CP010122:3845046-3845414 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYLAVYPTSETKK

Download         Length: 369 bp

>AT50501 NZ_CP010122:3845046-3845414 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCAGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L4J186

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4J164

References