Detailed information of TA system TA050499

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3764050..3764845
• Replicon: chromosome
• Accession: NZ_CP010122
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: RG28_RS19990
• Protein ID: WP_000854914.1
• Coordinates: 3764050..3764424 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L4J109
• Locus tag: RG28_RS19995
• Protein ID: WP_073521106.1
• Coordinates: 3764471..3764845 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS19955	3760722..3761426	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
RG28_RS19965	3761711..3761929	+	219	WP_001040187.1	translation initiation factor IF-1	-
RG28_RS19975	3762420..3763262	-	843	WP_073521104.1	DUF4942 domain-containing protein	-
RG28_RS19980	3763347..3763544	-	198	WP_073521105.1	DUF957 domain-containing protein	-
RG28_RS19985	3763562..3764053	-	492	WP_149027140.1	hypothetical protein	-
RG28_RS19990	3764050..3764424	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
RG28_RS19995	3764471..3764845	-	375	WP_073521106.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG28_RS20000	3764895..3765539	-	645	WP_061351931.1	hypothetical protein	-
RG28_RS20005	3765554..3765775	-	222	WP_000691817.1	DUF987 domain-containing protein	-
RG28_RS20010	3765844..3766320	-	477	WP_001186728.1	RadC family protein	-
RG28_RS20015	3766336..3766815	-	480	WP_061361461.1	antirestriction protein	-
RG28_RS20020	3766910..3767155	-	246	WP_000680583.1	hypothetical protein	-
RG28_RS20025	3767155..3767973	-	819	WP_061361460.1	DUF945 domain-containing protein	-
RG28_RS20040	3768257..3768712	-	456	WP_061361459.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T50499 WP_000854914.1 NZ_CP010122:c3764424-3764050 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T50499 NZ_CP010122:c3764424-3764050 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13644.34 Da
• Isoelectric Point: 5.9445

>AT50499 WP_073521106.1 NZ_CP010122:c3764845-3764471 [Escherichia coli]
VSDTFSGTTHPDDNNSRPWWGLPCTVTPCFGVRLVQEGNQLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT50499 NZ_CP010122:c3764845-3764471 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGATGACAACAACAGCCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTCGGCGTCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGTAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGATGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A1L4J109