Detailed information of TA system TA050498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3042504..3043142
• Replicon: chromosome
• Accession: NZ_CP010122
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: RG28_RS15825
• Protein ID: WP_000813794.1
• Coordinates: 3042966..3043142 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: RG28_RS15820
• Protein ID: WP_001270286.1
• Coordinates: 3042504..3042920 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS15800	3037656..3038597	-	942	WP_001251319.1	ABC transporter permease	-
RG28_RS15805	3038598..3039611	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
RG28_RS15810	3039629..3040774	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
RG28_RS15815	3041016..3042425	-	1410	WP_000760591.1	PLP-dependent aminotransferase family protein	-
RG28_RS15820	3042504..3042920	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
RG28_RS15825	3042966..3043142	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
RG28_RS15830	3043364..3043594	+	231	WP_000494244.1	YncJ family protein	-
RG28_RS15835	3043686..3045647	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
RG28_RS15840	3045720..3046256	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
RG28_RS15845	3046309..3047523	+	1215	WP_071830296.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3047563..3048711	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T50498 WP_000813794.1 NZ_CP010122:c3043142-3042966 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T50498 NZ_CP010122:c3043142-3042966 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT50498 WP_001270286.1 NZ_CP010122:c3042920-3042504 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT50498 NZ_CP010122:c3042920-3042504 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB