Detailed information of TA system TA050487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1901222..1901847
• Replicon: chromosome
• Accession: NZ_CP010122
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG28_RS09925
• Protein ID: WP_000911330.1
• Coordinates: 1901449..1901847 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: RG28_RS09920
• Protein ID: WP_000450524.1
• Coordinates: 1901222..1901449 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS09890	1897025..1897495	-	471	WP_073520940.1	thioredoxin-dependent thiol peroxidase	-
RG28_RS09895	1897495..1898067	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
RG28_RS09900	1898213..1899091	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
RG28_RS09905	1899108..1900142	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
RG28_RS09915	1900355..1901068	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RG28_RS09920	1901222..1901449	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG28_RS09925	1901449..1901847	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG28_RS09930	1901994..1902857	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
RG28_RS09935	1902872..1904887	+	2016	WP_021565358.1	tRNA cytosine(34) acetyltransferase TmcA	-
RG28_RS09940	1904961..1905659	+	699	WP_000679812.1	esterase	-
RG28_RS09945	1905779..1906759	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1905779..1906759	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T50487 WP_000911330.1 NZ_CP010122:1901449-1901847 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T50487 NZ_CP010122:1901449-1901847 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT50487 WP_000450524.1 NZ_CP010122:1901222-1901449 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT50487 NZ_CP010122:1901222-1901449 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33