Detailed information of TA system TA050481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 533217..534054
• Replicon: chromosome
• Accession: NZ_CP010122
• Organism: Escherichia coli strain C5

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: RG28_RS02850
• Protein ID: WP_000227784.1
• Coordinates: 533512..534054 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: RG28_RS02845
• Protein ID: WP_001297137.1
• Coordinates: 533217..533528 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG28_RS02820	528237..529184	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
RG28_RS02825	529206..531197	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
RG28_RS02830	531187..531801	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
RG28_RS02835	531801..532130	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
RG28_RS02840	532142..533032	+	891	WP_000971336.1	heme o synthase	-
RG28_RS02845	533217..533528	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
RG28_RS02850	533512..534054	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
RG28_RS02855	534110..534958	-	849	Protein_499	sel1 repeat family protein	-
RG28_RS02865	535008..536170	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
RG28_RS31065	536202..536306	-	105	Protein_501	sel1 repeat family protein	-
RG28_RS02875	536714..538078	+	1365	WP_001000974.1	MFS transporter	-
RG28_RS02880	538206..538697	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T50481 WP_000227784.1 NZ_CP010122:533512-534054 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T50481 NZ_CP010122:533512-534054 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT50481 WP_001297137.1 NZ_CP010122:533217-533528 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT50481 NZ_CP010122:533217-533528 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3