Detailed information of TA system TA050478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4707586..4708181
• Replicon: chromosome
• Accession: NZ_CP010121
• Organism: Escherichia coli strain C4

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: RG27_RS24150
• Protein ID: WP_000239581.1
• Coordinates: 4707586..4707936 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: RG27_RS24155
• Protein ID: WP_001223213.1
• Coordinates: 4707930..4708181 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG27_RS24130	4703033..4704055	-	1023	WP_001586590.1	ABC transporter permease	-
RG27_RS24135	4704069..4705571	-	1503	WP_000210560.1	sugar ABC transporter ATP-binding protein	-
RG27_RS24140	4705711..4706667	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
RG27_RS24145	4706977..4707507	+	531	WP_000055075.1	inorganic diphosphatase	-
RG27_RS24150	4707586..4707936	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
RG27_RS24155	4707930..4708181	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
RG27_RS24160	4708393..4708734	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
RG27_RS24165	4708737..4712516	-	3780	WP_000060861.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T50478 WP_000239581.1 NZ_CP010121:c4707936-4707586 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T50478 NZ_CP010121:c4707936-4707586 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT50478 WP_001223213.1 NZ_CP010121:c4708181-4707930 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT50478 NZ_CP010121:c4708181-4707930 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7