Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4397913..4398463 Replicon chromosome
Accession NZ_CP010121
Organism Escherichia coli strain C4

Toxin (Protein)


Gene name ccdB Uniprot ID A0A244BU83
Locus tag RG27_RS22600 Protein ID WP_001160962.1
Coordinates 4397913..4398227 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag RG27_RS22605 Protein ID WP_000125566.1
Coordinates 4398230..4398463 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG27_RS22575 4393588..4394874 + 1287 WP_000800470.1 peptidylprolyl isomerase SurA -
RG27_RS22580 4394874..4395863 + 990 WP_073461995.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
RG27_RS22585 4395860..4396681 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
RG27_RS22590 4396684..4397061 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
RG27_RS22595 4397068..4397916 + 849 WP_000257193.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
RG27_RS22600 4397913..4398227 - 315 WP_001160962.1 CcdB family protein Toxin
RG27_RS22605 4398230..4398463 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
RG27_RS22610 4398549..4399028 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
RG27_RS22615 4399220..4401082 - 1863 WP_000377114.1 glutathione-regulated potassium-efflux system protein KefC -
RG27_RS22620 4401075..4401605 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
RG27_RS22625 4401713..4403044 - 1332 WP_001183198.1 MFS transporter -
RG27_RS22630 4403103..4403390 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.79 Da        Isoelectric Point: 9.0851

>T50474 WP_001160962.1 NZ_CP010121:c4398227-4397913 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T50474 NZ_CP010121:c4398227-4397913 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGGGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT50474 WP_000125566.1 NZ_CP010121:c4398463-4398230 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT50474 NZ_CP010121:c4398463-4398230 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A244BU83

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References