Detailed information of TA system TA050470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3443404..3444109
• Replicon: chromosome
• Accession: NZ_CP010121
• Organism: Escherichia coli strain C4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: RG27_RS17805
• Protein ID: WP_000539521.1
• Coordinates: 3443404..3443790 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RG27_RS17810
• Protein ID: WP_001280945.1
• Coordinates: 3443780..3444109 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG27_RS17785	3439408..3440034	+	627	WP_001314584.1	glutathione S-transferase GstB	-
RG27_RS17790	3440031..3441146	-	1116	WP_000555033.1	aldose sugar dehydrogenase YliI	-
RG27_RS17795	3441257..3441640	-	384	WP_000497137.1	biofilm formation regulator BssR	-
RG27_RS17800	3441853..3443178	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
RG27_RS17805	3443404..3443790	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG27_RS17810	3443780..3444109	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
RG27_RS17815	3444179..3445507	-	1329	WP_073461946.1	GGDEF domain-containing protein	-
RG27_RS17820	3445515..3447863	-	2349	WP_073462049.1	EAL domain-containing protein	-
RG27_RS17825	3448041..3448952	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T50470 WP_000539521.1 NZ_CP010121:3443404-3443790 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T50470 NZ_CP010121:3443404-3443790 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT50470 WP_001280945.1 NZ_CP010121:3443780-3444109 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT50470 NZ_CP010121:3443780-3444109 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6