Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 588426..589057 Replicon chromosome
Accession NZ_CP010121
Organism Escherichia coli strain C4

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag RG27_RS02870 Protein ID WP_001260301.1
Coordinates 588426..588701 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag RG27_RS02875 Protein ID WP_000593555.1
Coordinates 588698..589057 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG27_RS02855 583430..584497 + 1068 WP_000361493.1 HlyD family efflux transporter periplasmic adaptor subunit -
RG27_RS02860 584494..587229 + 2736 WP_073461816.1 ribosome-associated ATPase/putative transporter RbbA -
RG27_RS02865 587229..588353 + 1125 WP_001315056.1 ABC transporter permease -
RG27_RS02870 588426..588701 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
RG27_RS02875 588698..589057 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
RG27_RS02880 589177..589578 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
RG27_RS02885 589583..590389 - 807 WP_000173658.1 nickel import ATP-binding protein NikE -
RG27_RS02890 590386..591150 - 765 WP_001136198.1 nickel import ATP-binding protein NikD -
RG27_RS02895 591150..591983 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
RG27_RS02900 591980..592924 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 581442..589578 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T50453 WP_001260301.1 NZ_CP010121:588426-588701 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T50453 NZ_CP010121:588426-588701 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT50453 WP_000593555.1 NZ_CP010121:588698-589057 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT50453 NZ_CP010121:588698-589057 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References