Detailed information of TA system TA050450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 33390..34015
• Replicon: plasmid A
• Accession: NZ_CP010120
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: RG26_RS29230
• Protein ID: WP_000911317.1
• Coordinates: 33617..34015 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: RG26_RS29225
• Protein ID: WP_000450532.1
• Coordinates: 33390..33617 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS29225	33390..33617	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
RG26_RS29230	33617..34015	+	399	WP_000911317.1	tRNA(fMet)-specific endonuclease VapC	Toxin
RG26_RS29235	34024..36177	-	2154	WP_040092615.1	type IV conjugative transfer system coupling protein TraD	-
RG26_RS29240	36430..37161	-	732	WP_024248462.1	complement resistance protein TraT	-
RG26_RS29245	37210..37695	-	486	WP_077827312.1	surface exclusion protein	-
RG26_RS29265	37768..38465	+	698	Protein_47	IS1-like element IS1A family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeH / faeF / faeE / faeD / faeC	1..63684	63684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T50450 WP_000911317.1 NZ_CP010120:33617-34015 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T50450 NZ_CP010120:33617-34015 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGGCTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT50450 WP_000450532.1 NZ_CP010120:33390-33617 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT50450 NZ_CP010120:33390-33617 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGCGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5