Detailed information of TA system TA050447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 24378..25153
• Replicon: plasmid A
• Accession: NZ_CP010120
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8D9PPA8
• Locus tag: RG26_RS29185
• Protein ID: WP_000405244.1
• Coordinates: 24378..24860 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: RG26_RS29190
• Protein ID: WP_001346256.1
• Coordinates: 24851..25153 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS29135	19529..19879	+	351	Protein_27	transposase family protein	-
RG26_RS29140	19876..20628	-	753	Protein_28	DUF4158 domain-containing protein	-
RG26_RS29145	20753..21028	-	276	WP_000421253.1	type II toxin-antitoxin system RelE/ParE family toxin	-
RG26_RS29150	21028..21312	-	285	WP_024182491.1	ribbon-helix-helix protein, CopG family	-
RG26_RS29160	22178..23035	-	858	WP_000774299.1	incFII family plasmid replication initiator RepA	-
RG26_RS29165	23028..23510	-	483	WP_000247130.1	hypothetical protein	-
RG26_RS29170	23503..23577	-	75	WP_032164225.1	RepA leader peptide Tap	-
RG26_RS29180	23812..24069	-	258	WP_000083826.1	replication regulatory protein RepA	-
RG26_RS29185	24378..24860	-	483	WP_000405244.1	GNAT family N-acetyltransferase	Toxin
RG26_RS29190	24851..25153	-	303	WP_001346256.1	DUF1778 domain-containing protein	Antitoxin
RG26_RS29195	25475..25681	-	207	WP_032232098.1	type I toxin-antitoxin system Hok family toxin	-
-	25668..25729	+	62	NuclAT_1	-	-
-	25668..25729	+	62	NuclAT_1	-	-
-	25668..25729	+	62	NuclAT_1	-	-
-	25668..25729	+	62	NuclAT_1	-	-
RG26_RS29205	26313..26525	-	213	WP_032323685.1	hypothetical protein	-
RG26_RS29210	26657..27217	-	561	WP_024244031.1	fertility inhibition protein FinO	-
RG26_RS29215	27272..28018	-	747	WP_000205747.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeH / faeF / faeE / faeD / faeC	1..63684	63684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-99)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17659.41 Da
• Isoelectric Point: 7.1036

>T50447 WP_000405244.1 NZ_CP010120:c24860-24378 [Escherichia coli]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T50447 NZ_CP010120:c24860-24378 [Escherichia coli]
ATGGAAATAAATGTCACCGCACCGGCATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
GAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGGTACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11602.19 Da
• Isoelectric Point: 4.4569

>AT50447 WP_001346256.1 NZ_CP010120:c25153-24851 [Escherichia coli]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT50447 NZ_CP010120:c25153-24851 [Escherichia coli]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCTGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAACTGGCTTACCAGG
AAGCTAAAAACATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA