Detailed information of TA system TA050446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 20753..21312
• Replicon: plasmid A
• Accession: NZ_CP010120
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: RG26_RS29145
• Protein ID: WP_000421253.1
• Coordinates: 20753..21028 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RG26_RS29150
• Protein ID: WP_024182491.1
• Coordinates: 21028..21312 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS29090	15848..16438	-	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
RG26_RS29100	16790..17173	+	384	WP_001063020.1	relaxosome protein TraM	-
RG26_RS29105	17341..18038	-	698	Protein_24	IS1-like element IS1A family transposase	-
RG26_RS29125	18755..18955	+	201	WP_024182490.1	hypothetical protein	-
RG26_RS29130	19060..19427	+	368	Protein_26	transposase	-
RG26_RS29135	19529..19879	+	351	Protein_27	transposase family protein	-
RG26_RS29140	19876..20628	-	753	Protein_28	DUF4158 domain-containing protein	-
RG26_RS29145	20753..21028	-	276	WP_000421253.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RG26_RS29150	21028..21312	-	285	WP_024182491.1	ribbon-helix-helix protein, CopG family	Antitoxin
RG26_RS29160	22178..23035	-	858	WP_000774299.1	incFII family plasmid replication initiator RepA	-
RG26_RS29165	23028..23510	-	483	WP_000247130.1	hypothetical protein	-
RG26_RS29170	23503..23577	-	75	WP_032164225.1	RepA leader peptide Tap	-
RG26_RS29180	23812..24069	-	258	WP_000083826.1	replication regulatory protein RepA	-
RG26_RS29185	24378..24860	-	483	WP_000405244.1	GNAT family N-acetyltransferase	-
RG26_RS29190	24851..25153	-	303	WP_001346256.1	DUF1778 domain-containing protein	-
RG26_RS29195	25475..25681	-	207	WP_032232098.1	type I toxin-antitoxin system Hok family toxin	-
-	25668..25729	+	62	NuclAT_1	-	-
-	25668..25729	+	62	NuclAT_1	-	-
-	25668..25729	+	62	NuclAT_1	-	-
-	25668..25729	+	62	NuclAT_1	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeH / faeF / faeE / faeD / faeC	1..63684	63684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10755.41 Da
• Isoelectric Point: 9.5302

>T50446 WP_000421253.1 NZ_CP010120:c21028-20753 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPLILLTHPRMGEQLFQFDPREVRRFFAGEYEIRYELNGQTIY
VLRLWHTQENR

Nucleotide

• Length: 276 bp

>T50446 NZ_CP010120:c21028-20753 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTACTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTGCAGTCCCTGACACAGGCACCGCTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGACCCCAGGGAGGTCAGACGGTTTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGGCTGTGGCACACACAAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10497.98 Da
• Isoelectric Point: 6.7244

>AT50446 WP_024182491.1 NZ_CP010120:c21312-21028 [Escherichia coli]
MRMKNNSAQATKVITAHVPLLMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLGTDHPLPVPR

Nucleotide

• Length: 285 bp

>AT50446 NZ_CP010120:c21312-21028 [Escherichia coli]
ATGCGAATGAAAAACAATAGTGCACAAGCAACAAAAGTAATTACCGCACATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCTCGGGGGTGGGTTATTAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGATCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGGTACTGACCATCCGTTACCGGTGCCACGCTGA