Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 4770042..4770523 Replicon chromosome
Accession NZ_CP010119
Organism Escherichia coli strain C3

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7YS45
Locus tag RG26_RS25570 Protein ID WP_014966210.1
Coordinates 4770042..4770332 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID A0A0D7Y007
Locus tag RG26_RS25575 Protein ID WP_000100896.1
Coordinates 4770332..4770523 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG26_RS25530 4765399..4765668 - 270 WP_000003742.1 excisionase -
RG26_RS25535 4765730..4768201 - 2472 WP_073519354.1 exonuclease -
RG26_RS25540 4768294..4768485 - 192 WP_001090200.1 DUF1482 family protein -
RG26_RS25545 4768482..4768670 - 189 WP_000449192.1 cell division inhibition protein DicB -
RG26_RS25550 4769071..4769235 + 165 WP_001331716.1 hypothetical protein -
RG26_RS25555 4769239..4769457 - 219 WP_001171974.1 hypothetical protein -
RG26_RS25565 4769617..4769772 - 156 WP_000379575.1 DUF1391 family protein -
RG26_RS25570 4770042..4770332 - 291 WP_014966210.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RG26_RS25575 4770332..4770523 - 192 WP_000100896.1 hypothetical protein Antitoxin
RG26_RS25580 4770541..4771041 - 501 WP_000823909.1 helix-turn-helix transcriptional regulator -
RG26_RS25585 4771149..4771424 + 276 WP_001048458.1 helix-turn-helix domain-containing protein -
RG26_RS25590 4771408..4771833 + 426 WP_000693918.1 Rha family transcriptional regulator -
RG26_RS30570 4771905..4772921 + 1017 WP_001262344.1 hypothetical protein -
RG26_RS30575 4772833..4773375 + 543 WP_072130322.1 replication protein -
RG26_RS25605 4773409..4774179 + 771 WP_000450649.1 DUF1627 domain-containing protein -
RG26_RS25610 4774195..4774617 + 423 WP_001151239.1 DUF977 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espW / espM2 4757143..4818532 61389
- flank IS/Tn - - 4774696..4775676 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11176.05 Da        Isoelectric Point: 9.9854

>T50433 WP_014966210.1 NZ_CP010119:c4770332-4770042 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKTC

Download         Length: 291 bp

>T50433 NZ_CP010119:c4770332-4770042 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGATGATTTACGTCAGATCGTAGCCTATATTGCTAAGGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGTGAGATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAGCATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGACAGTATCCAAACAAAACCTGTTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7033.83 Da        Isoelectric Point: 4.0270

>AT50433 WP_000100896.1 NZ_CP010119:c4770523-4770332 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRTKVAASLSDPRPAIPHDEVMAEMENLIAQLAATNRSE

Download         Length: 192 bp

>AT50433 NZ_CP010119:c4770523-4770332 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACAGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAAC
CAAAGTGGCAGCAAGCCTCTCAGATCCCCGTCCTGCAATTCCACATGACGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTGCAACGAACAGGAGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7YS45

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7Y007

References