Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2643066..2643720 Replicon chromosome
Accession NZ_CP010119
Organism Escherichia coli strain C3

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag RG26_RS13870 Protein ID WP_000244772.1
Coordinates 2643313..2643720 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG26_RS13865 Protein ID WP_000354046.1
Coordinates 2643066..2643332 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG26_RS13845 2639354..2639665 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
RG26_RS13850 2639829..2640488 + 660 WP_000250274.1 hemolysin III family protein -
RG26_RS13855 2640611..2641591 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
RG26_RS13860 2641843..2642823 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
RG26_RS13865 2643066..2643332 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG26_RS13870 2643313..2643720 + 408 WP_000244772.1 protein YgfX Toxin
RG26_RS13875 2643760..2644281 - 522 WP_001055874.1 flavodoxin FldB -
RG26_RS13880 2644393..2645289 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG26_RS13885 2645314..2646024 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG26_RS13890 2646030..2647763 + 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2640611..2641591 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T50420 WP_000244772.1 NZ_CP010119:2643313-2643720 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50420 NZ_CP010119:2643313-2643720 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50420 WP_000354046.1 NZ_CP010119:2643066-2643332 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50420 NZ_CP010119:2643066-2643332 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References