Detailed information of TA system TA050412

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 969354..970129
• Replicon: chromosome
• Accession: NZ_CP010119
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A7V7JG91
• Locus tag: RG26_RS05045
• Protein ID: WP_001193488.1
• Coordinates: 969354..969725 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7V7JFU6
• Locus tag: RG26_RS05050
• Protein ID: WP_001059301.1
• Coordinates: 969764..970129 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS05020	965312..966028	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
RG26_RS05025	966048..967154	+	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
RG26_RS05030	967219..968199	+	981	WP_000991444.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
RG26_RS05045	969354..969725	-	372	WP_001193488.1	TA system toxin CbtA family protein	Toxin
RG26_RS05050	969764..970129	-	366	WP_001059301.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RG26_RS05055	970155..970376	-	222	WP_000691983.1	DUF987 domain-containing protein	-
RG26_RS05060	970373..970915	-	543	WP_015740440.1	DNA repair protein RadC	-
RG26_RS05065	970928..971371	-	444	WP_001096016.1	antirestriction protein	-
RG26_RS05070	971402..972223	-	822	WP_001234409.1	DUF945 domain-containing protein	-
RG26_RS05075	972343..972816	-	474	WP_001298943.1	hypothetical protein	-
RG26_RS05080	972888..973340	-	453	WP_000734321.1	hypothetical protein	-
RG26_RS05085	973376..974092	-	717	WP_000174910.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	959785..976870	17085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13832.94 Da
• Isoelectric Point: 7.2419

>T50412 WP_001193488.1 NZ_CP010119:c969725-969354 [Escherichia coli]
MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFSNETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDC
SVMEKSPFITSIDILRARKASGLMKRNSHKTVTRVTAGRLQEL

Nucleotide

• Length: 372 bp

>T50412 NZ_CP010119:c969725-969354 [Escherichia coli]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACTGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.9530

>AT50412 WP_001059301.1 NZ_CP010119:c970129-969764 [Escherichia coli]
MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLSDRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHPHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT50412 NZ_CP010119:c970129-969764 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A7V7JG91

Antitoxin

• AlphaFold DB: A0A7V7JFU6