Detailed information of TA system TA050410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 412023..412860
• Replicon: chromosome
• Accession: NZ_CP010119
• Organism: Escherichia coli strain C3

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: RG26_RS02285
• Protein ID: WP_073519256.1
• Coordinates: 412318..412860 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: RG26_RS02280
• Protein ID: WP_001297137.1
• Coordinates: 412023..412334 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RG26_RS02255	407043..407990	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
RG26_RS02260	408012..410003	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
RG26_RS02265	409993..410607	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
RG26_RS02270	410607..410936	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
RG26_RS02275	410948..411838	+	891	WP_000971336.1	heme o synthase	-
RG26_RS02280	412023..412334	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
RG26_RS02285	412318..412860	+	543	WP_073519256.1	GNAT family N-acetyltransferase	Toxin
RG26_RS02290	412916..413851	-	936	WP_001297127.1	sel1 repeat family protein	-
RG26_RS02295	414259..415623	+	1365	WP_001000978.1	MFS transporter	-
RG26_RS02300	415751..416242	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
RG26_RS02305	416410..417321	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19810.98 Da
• Isoelectric Point: 8.0330

>T50410 WP_073519256.1 NZ_CP010119:412318-412860 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQFSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T50410 NZ_CP010119:412318-412860 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCAGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGTTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT50410 WP_001297137.1 NZ_CP010119:412023-412334 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT50410 NZ_CP010119:412023-412334 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3