Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4437723..4438377 Replicon chromosome
Accession NZ_CP010117
Organism Escherichia coli strain C2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag RG25_RS22935 Protein ID WP_000244781.1
Coordinates 4437970..4438377 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RG25_RS22930 Protein ID WP_000354046.1
Coordinates 4437723..4437989 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG25_RS22905 4432892..4433635 + 744 WP_000951951.1 SDR family oxidoreductase -
RG25_RS22910 4433692..4435125 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
RG25_RS22915 4435170..4435481 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
RG25_RS22920 4435645..4436304 + 660 WP_000250274.1 hemolysin III family protein -
RG25_RS22925 4436500..4437480 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
RG25_RS22930 4437723..4437989 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RG25_RS22935 4437970..4438377 + 408 WP_000244781.1 protein YgfX Toxin
RG25_RS22940 4438417..4438938 - 522 WP_001055874.1 flavodoxin FldB -
RG25_RS22945 4439050..4439946 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
RG25_RS22950 4439971..4440681 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RG25_RS22955 4440687..4442420 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T50407 WP_000244781.1 NZ_CP010117:4437970-4438377 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50407 NZ_CP010117:4437970-4438377 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50407 WP_000354046.1 NZ_CP010117:4437723-4437989 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50407 NZ_CP010117:4437723-4437989 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References