Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1231141..1231779 Replicon chromosome
Accession NZ_CP010117
Organism Escherichia coli strain C2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RG25_RS06310 Protein ID WP_000813794.1
Coordinates 1231603..1231779 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RG25_RS06305 Protein ID WP_001270285.1
Coordinates 1231141..1231557 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG25_RS06285 1226293..1227234 - 942 WP_001251315.1 ABC transporter permease -
RG25_RS06290 1227235..1228248 - 1014 WP_000220411.1 ABC transporter ATP-binding protein -
RG25_RS06295 1228266..1229411 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
RG25_RS06300 1229656..1231062 - 1407 WP_000760594.1 PLP-dependent aminotransferase family protein -
RG25_RS06305 1231141..1231557 - 417 WP_001270285.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RG25_RS06310 1231603..1231779 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RG25_RS06315 1232001..1232231 + 231 WP_000494244.1 YncJ family protein -
RG25_RS06320 1232323..1234284 - 1962 WP_032255020.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RG25_RS06325 1234357..1234893 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
RG25_RS06330 1234946..1236160 + 1215 WP_071601296.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(88-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T50399 WP_000813794.1 NZ_CP010117:c1231779-1231603 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T50399 NZ_CP010117:c1231779-1231603 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15247.61 Da        Isoelectric Point: 4.6115

>AT50399 WP_001270285.1 NZ_CP010117:c1231557-1231141 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT50399 NZ_CP010117:c1231557-1231141 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References