Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4423390..4424044 Replicon chromosome
Accession NZ_CP010116
Organism Escherichia coli strain C1

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag RC72_RS22770 Protein ID WP_000244781.1
Coordinates 4423390..4423797 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag RC72_RS22775 Protein ID WP_000354046.1
Coordinates 4423778..4424044 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RC72_RS22750 4419347..4421080 - 1734 WP_001598594.1 single-stranded-DNA-specific exonuclease RecJ -
RC72_RS22755 4421086..4421796 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
RC72_RS22760 4421821..4422717 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
RC72_RS22765 4422829..4423350 + 522 WP_001055874.1 flavodoxin FldB -
RC72_RS22770 4423390..4423797 - 408 WP_000244781.1 protein YgfX Toxin
RC72_RS22775 4423778..4424044 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
RC72_RS22780 4424287..4425267 + 981 WP_001562036.1 tRNA-modifying protein YgfZ -
RC72_RS22785 4425344..4426003 - 660 WP_000250269.1 hemolysin III family protein -
RC72_RS22790 4426167..4426478 - 312 WP_001598596.1 N(4)-acetylcytidine aminohydrolase -
RC72_RS22795 4426523..4427956 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T50387 WP_000244781.1 NZ_CP010116:c4423797-4423390 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T50387 NZ_CP010116:c4423797-4423390 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT50387 WP_000354046.1 NZ_CP010116:c4424044-4423778 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT50387 NZ_CP010116:c4424044-4423778 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References