Detailed information of TA system TA050367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2105486..2106191
• Replicon: chromosome
• Accession: NZ_CP010116
• Organism: Escherichia coli strain C1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: RC72_RS10500
• Protein ID: WP_000539521.1
• Coordinates: 2105805..2106191 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RC72_RS10495
• Protein ID: WP_001280945.1
• Coordinates: 2105486..2105815 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC72_RS10480	2100643..2101554	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
RC72_RS10485	2101732..2104080	+	2349	WP_001328220.1	EAL domain-containing protein	-
RC72_RS10490	2104088..2105416	+	1329	WP_049068068.1	GGDEF domain-containing protein	-
RC72_RS10495	2105486..2105815	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
RC72_RS10500	2105805..2106191	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RC72_RS10505	2106417..2107742	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
RC72_RS10510	2107955..2108338	+	384	WP_000497137.1	biofilm formation regulator BssR	-
RC72_RS10515	2108449..2109564	+	1116	WP_001595547.1	aldose sugar dehydrogenase YliI	-
RC72_RS10520	2109561..2110187	-	627	WP_001595548.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T50367 WP_000539521.1 NZ_CP010116:c2106191-2105805 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T50367 NZ_CP010116:c2106191-2105805 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT50367 WP_001280945.1 NZ_CP010116:c2105815-2105486 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT50367 NZ_CP010116:c2105815-2105486 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6