Detailed information of TA system TA050361

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1173750..1174585
• Replicon: chromosome
• Accession: NZ_CP010116
• Organism: Escherichia coli strain C1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LDL9
• Locus tag: RC72_RS05995
• Protein ID: WP_001094444.1
• Coordinates: 1174208..1174585 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7LDN9
• Locus tag: RC72_RS05990
• Protein ID: WP_001285607.1
• Coordinates: 1173750..1174118 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RC72_RS05960	1168880..1169626	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
RC72_RS05965	1169641..1171182	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
RC72_RS05970	1171476..1172294	+	819	WP_001175175.1	DUF945 domain-containing protein	-
RC72_RS05975	1172386..1172871	+	486	WP_000213722.1	antirestriction protein	-
RC72_RS05980	1172886..1173362	+	477	WP_001384029.1	RadC family protein	-
RC72_RS05985	1173449..1173670	+	222	WP_000692350.1	DUF987 domain-containing protein	-
RC72_RS05990	1173750..1174118	+	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RC72_RS05995	1174208..1174585	+	378	WP_001094444.1	TA system toxin CbtA family protein	Toxin
RC72_RS06000	1174582..1175070	+	489	WP_000761685.1	hypothetical protein	-
RC72_RS06005	1175090..1175287	+	198	WP_001327226.1	DUF957 domain-containing protein	-
RC72_RS25935	1175372..1175518	+	147	WP_001290178.1	hypothetical protein	-
RC72_RS06015	1176527..1177870	+	1344	WP_072135398.1	hypothetical protein	-
RC72_RS06020	1177982..1178563	+	582	WP_072135395.1	hypothetical protein	-
RC72_RS06025	1178962..1179141	-	180	WP_001315171.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1138896..1188716	49820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14087.02 Da
• Isoelectric Point: 7.3523

>T50361 WP_001094444.1 NZ_CP010116:1174208-1174585 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SVCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T50361 NZ_CP010116:1174208-1174585 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTACTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13689.53 Da
• Isoelectric Point: 6.4669

>AT50361 WP_001285607.1 NZ_CP010116:1173750-1174118 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT50361 NZ_CP010116:1173750-1174118 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: B1LDL9

Antitoxin

• AlphaFold DB: A0A1M0JLU8