Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 1954866..1955530 Replicon chromosome
Accession NZ_CP010114
Organism Mycobacterium avium subsp. paratuberculosis strain E93

Toxin (Protein)


Gene name vapC Uniprot ID Q73YC9
Locus tag RE97_RS08990 Protein ID WP_003872398.1
Coordinates 1955123..1955530 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q73YC8
Locus tag RE97_RS08985 Protein ID WP_003872399.1
Coordinates 1954866..1955126 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RE97_RS08965 1950282..1950944 + 663 WP_003872403.1 TetR/AcrR family transcriptional regulator -
RE97_RS08970 1951140..1952597 + 1458 WP_003878220.1 serine/threonine protein kinase -
RE97_RS08975 1952799..1954067 - 1269 WP_003872401.1 cytochrome P450 -
RE97_RS08980 1954233..1954685 + 453 WP_003872400.1 VOC family protein -
RE97_RS08985 1954866..1955126 + 261 WP_003872399.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RE97_RS08990 1955123..1955530 + 408 WP_003872398.1 type II toxin-antitoxin system VapC family toxin Toxin
RE97_RS22745 1955677..1957221 - 1545 WP_010949452.1 serine/threonine protein kinase -
RE97_RS08995 1957458..1957979 + 522 WP_003878215.1 carboxymuconolactone decarboxylase family protein -
RE97_RS09000 1957976..1958521 + 546 WP_003878214.1 carboxymuconolactone decarboxylase family protein -
RE97_RS09005 1958642..1959172 + 531 WP_003878213.1 TetR/AcrR family transcriptional regulator -
RE97_RS09010 1959161..1960330 - 1170 WP_003878212.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-64)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14418.47 Da        Isoelectric Point: 4.5642

>T50355 WP_003872398.1 NZ_CP010114:1955123-1955530 [Mycobacterium avium subsp. paratuberculosis]
MTARPAAGVLDTSVFIASESGRQLDEARIPDEVATTVVTLAELHAGVLAATTSNVRAQRLATLESIADMEMLPVDDDAVR
MWARLRIHLAEAGRRMRANDLWIAAIAASRGLPVVTQDDDFAALDGAANLAIIRV

Download         Length: 408 bp

>T50355 NZ_CP010114:1955123-1955530 [Mycobacterium avium subsp. paratuberculosis]
ATGACTGCAAGGCCGGCGGCCGGGGTGCTCGACACCTCGGTGTTCATCGCGTCCGAAAGCGGACGGCAGCTGGACGAGGC
GCGTATCCCCGACGAGGTAGCCACCACCGTCGTCACCCTTGCCGAACTTCACGCCGGCGTATTAGCCGCGACGACGTCCA
ACGTTCGGGCGCAACGCCTCGCAACGCTGGAATCGATAGCGGATATGGAAATGCTGCCAGTCGACGACGACGCGGTCCGC
ATGTGGGCAAGGTTGCGGATCCACCTAGCCGAGGCTGGACGGCGGATGCGGGCTAATGATCTGTGGATCGCGGCGATCGC
GGCATCGCGGGGACTGCCCGTTGTCACCCAGGATGACGACTTCGCCGCACTCGACGGCGCGGCGAACCTGGCGATTATTC
GGGTTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9635.01 Da        Isoelectric Point: 11.7137

>AT50355 WP_003872399.1 NZ_CP010114:1954866-1955126 [Mycobacterium avium subsp. paratuberculosis]
MSEVASRELRNDTAGVLRRVREGEDVTITINGRAVAMLTPLRPQRRRWLSKAELLSRLRRAQADPGLRQDLATLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT50355 NZ_CP010114:1954866-1955126 [Mycobacterium avium subsp. paratuberculosis]
ATGTCTGAAGTGGCGTCTCGCGAGCTGCGCAACGATACGGCGGGGGTACTGCGGCGCGTACGGGAAGGGGAGGACGTCAC
CATCACCATCAACGGCCGAGCCGTCGCGATGCTGACCCCGCTGCGGCCGCAGCGACGGCGGTGGCTGAGCAAGGCGGAGT
TGCTGTCGCGCTTGCGCCGCGCGCAAGCCGATCCCGGGCTACGCCAAGACCTCGCAACCCTTGCAGGCGACACCACTGAG
GACCTCGGTCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L5MIC8

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MIC5

References