Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1187400..1187964 Replicon chromosome
Accession NZ_CP010114
Organism Mycobacterium avium subsp. paratuberculosis strain E93

Toxin (Protein)


Gene name vapC Uniprot ID X7VBD8
Locus tag RE97_RS05640 Protein ID WP_003875391.1
Coordinates 1187593..1187964 (+) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7L5MGM2
Locus tag RE97_RS22630 Protein ID WP_003875390.1
Coordinates 1187400..1187606 (+) Length 69 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RE97_RS05620 1182654..1183652 + 999 WP_003875386.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
RE97_RS05625 1183676..1184883 - 1208 Protein_1134 hypothetical protein -
RE97_RS05630 1185014..1186087 + 1074 WP_010949695.1 redox-regulated ATPase YchF -
RE97_RS05635 1186287..1187231 + 945 WP_003875389.1 HNH endonuclease -
RE97_RS22630 1187400..1187606 + 207 WP_003875390.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
RE97_RS05640 1187593..1187964 + 372 WP_003875391.1 type II toxin-antitoxin system VapC family toxin Toxin
RE97_RS05645 1188064..1188504 + 441 WP_010949694.1 nitroreductase family deazaflavin-dependent oxidoreductase -
RE97_RS05650 1188560..1188835 + 276 WP_003878531.1 hypothetical protein -
RE97_RS05655 1188937..1189335 + 399 WP_003875394.1 VOC family protein -
RE97_RS22635 1189402..1189785 + 384 WP_003875395.1 hypothetical protein -
RE97_RS05660 1189855..1190208 + 354 WP_003875396.1 hypothetical protein -
RE97_RS05665 1190365..1190889 + 525 WP_033713819.1 glycoside hydrolase -
RE97_RS05670 1191300..1191624 + 325 Protein_1145 antibiotic biosynthesis monooxygenase -
RE97_RS05675 1191629..1192312 - 684 WP_003875399.1 hypothetical protein -
RE97_RS05680 1192430..1192627 + 198 WP_016706054.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13770.14 Da        Isoelectric Point: 6.6003

>T50354 WP_003875391.1 NZ_CP010114:1187593-1187964 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Download         Length: 372 bp

>T50354 NZ_CP010114:1187593-1187964 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin


Download         Length: 69 a.a.        Molecular weight: 7231.22 Da        Isoelectric Point: 11.0634

>AT50354 WP_003875390.1 NZ_CP010114:1187400-1187606 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Download         Length: 207 bp

>AT50354 NZ_CP010114:1187400-1187606 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2WB37

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MGM2

References