Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 229368..230010 Replicon chromosome
Accession NZ_CP010106
Organism Bacillus thuringiensis serovar indiana strain HD521

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag NF53_RS01275 Protein ID WP_000635965.1
Coordinates 229660..230010 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag NF53_RS01270 Protein ID WP_000004570.1
Coordinates 229368..229655 (+) Length 96 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NF53_RS01245 224685..225647 + 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -
NF53_RS01250 225640..226212 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
NF53_RS01255 226306..226665 + 360 WP_000583417.1 holo-ACP synthase -
NF53_RS01260 226822..227772 + 951 WP_033689732.1 outer membrane lipoprotein carrier protein LolA -
NF53_RS01265 227890..229059 + 1170 WP_000390615.1 alanine racemase -
NF53_RS01270 229368..229655 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
NF53_RS01275 229660..230010 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NF53_RS01280 230078..232246 + 2169 WP_044306890.1 RNA-binding transcriptional accessory protein -
NF53_RS01285 232304..232420 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
NF53_RS01290 232616..233074 + 459 WP_003311668.1 SprT family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T50352 WP_000635965.1 NZ_CP010106:229660-230010 [Bacillus thuringiensis serovar indiana]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T50352 NZ_CP010106:229660-230010 [Bacillus thuringiensis serovar indiana]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT50352 WP_000004570.1 NZ_CP010106:229368-229655 [Bacillus thuringiensis serovar indiana]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT50352 NZ_CP010106:229368-229655 [Bacillus thuringiensis serovar indiana]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A366G118


Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References