Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2185335..2185977 Replicon chromosome
Accession NZ_CP010088
Organism Bacillus thuringiensis strain 97-27

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BG06_RS11680 Protein ID WP_000635963.1
Coordinates 2185335..2185685 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BG06_RS11685 Protein ID WP_000004570.1
Coordinates 2185690..2185977 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG06_RS11665 2182271..2182729 - 459 WP_000344247.1 SprT family protein -
BG06_RS11670 2182925..2183041 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BG06_RS11675 2183099..2185267 - 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BG06_RS11680 2185335..2185685 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BG06_RS11685 2185690..2185977 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BG06_RS11690 2186287..2187456 - 1170 WP_000390600.1 alanine racemase -
BG06_RS11695 2187575..2188525 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BG06_RS11700 2188682..2189041 - 360 WP_000635040.1 holo-ACP synthase -
BG06_RS11705 2189134..2189706 + 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BG06_RS11710 2189699..2190661 - 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T50349 WP_000635963.1 NZ_CP010088:c2185685-2185335 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T50349 NZ_CP010088:c2185685-2185335 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT50349 WP_000004570.1 NZ_CP010088:c2185977-2185690 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT50349 NZ_CP010088:c2185977-2185690 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References