Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 66092..67067 Replicon chromosome
Accession NZ_CP010088
Organism Bacillus thuringiensis strain 97-27

Toxin (Protein)


Gene name spoIISA Uniprot ID C3GJ28
Locus tag BG06_RS00735 Protein ID WP_003297560.1
Coordinates 66330..67067 (-) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag BG06_RS00730 Protein ID WP_000588712.1
Coordinates 66092..66217 (-) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG06_RS00700 61152..61979 - 828 WP_001060391.1 hypothetical protein -
BG06_RS00705 61969..62502 - 534 WP_000438338.1 sigma-70 family RNA polymerase sigma factor -
BG06_RS00710 62724..63344 - 621 WP_144405038.1 SGNH/GDSL hydrolase family protein -
BG06_RS00715 63521..63991 + 471 WP_000670597.1 DUF5065 family protein -
BG06_RS00720 64226..65695 - 1470 WP_000287514.1 beta-Ala-His dipeptidase -
BG06_RS29925 65839..66015 - 177 WP_000808045.1 hypothetical protein -
BG06_RS00730 66092..66217 - 126 WP_000588712.1 hypothetical protein Antitoxin
BG06_RS00735 66330..67067 - 738 WP_003297560.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
BG06_RS00740 67256..67993 - 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
BG06_RS00745 68161..68643 - 483 WP_000191912.1 MarR family winged helix-turn-helix transcriptional regulator -
BG06_RS00750 68750..70435 + 1686 WP_003297558.1 alpha-keto acid decarboxylase family protein -
BG06_RS00755 70557..71339 - 783 WP_000381788.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28373.07 Da        Isoelectric Point: 8.2672

>T50348 WP_003297560.1 NZ_CP010088:c67067-66330 [Bacillus thuringiensis]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T50348 NZ_CP010088:c67067-66330 [Bacillus thuringiensis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACTGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT50348 WP_000588712.1 NZ_CP010088:c66217-66092 [Bacillus thuringiensis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT50348 NZ_CP010088:c66217-66092 [Bacillus thuringiensis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9EK80

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References