Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518415..519051 Replicon chromosome
Accession NZ_CP010053
Organism Bacillus subtilis strain PS832

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag QX56_RS02580 Protein ID WP_003156187.1
Coordinates 518701..519051 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag QX56_RS02575 Protein ID WP_003225183.1
Coordinates 518415..518696 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QX56_RS02555 514775..515374 - 600 WP_003225175.1 rhomboid family intramembrane serine protease -
QX56_RS02560 515469..515834 + 366 WP_003225177.1 holo-ACP synthase -
QX56_RS02565 515999..517015 + 1017 WP_080273356.1 outer membrane lipoprotein carrier protein LolA -
QX56_RS02570 517130..518299 + 1170 WP_003234284.1 alanine racemase -
QX56_RS02575 518415..518696 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
QX56_RS02580 518701..519051 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
QX56_RS02585 519166..519990 + 825 WP_080314625.1 RsbT co-antagonist protein RsbRA -
QX56_RS02590 519995..520360 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
QX56_RS02595 520364..520765 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
QX56_RS02600 520777..521784 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
QX56_RS02605 521846..522175 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
QX56_RS02610 522172..522654 + 483 WP_003234299.1 anti-sigma B factor RsbW -
QX56_RS02615 522620..523408 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
QX56_RS02620 523408..524007 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T50300 WP_003156187.1 NZ_CP010053:518701-519051 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T50300 NZ_CP010053:518701-519051 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT50300 WP_003225183.1 NZ_CP010053:518415-518696 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT50300 NZ_CP010053:518415-518696 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References