50278

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1348454..1349370 Replicon chromosome
Accession NZ_CP010052
Organism Bacillus subtilis subsp. subtilis str. 168

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag QU35_RS06890 Protein ID WP_003244695.1
Coordinates 1348624..1349370 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag QU35_RS06885 Protein ID WP_003232646.1
Coordinates 1348454..1348624 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QU35_RS06855 1345317..1345646 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
QU35_RS06860 1345643..1345807 + 165 WP_003232658.1 XkdX family protein -
QU35_RS06865 1345851..1346690 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
QU35_RS06870 1346743..1347012 + 270 WP_003232655.1 hemolysin XhlA family protein -
QU35_RS06875 1347025..1347288 + 264 WP_003232653.1 phage holin -
QU35_RS06880 1347301..1348194 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
QU35_RS22380 1348231..1348368 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
QU35_RS06885 1348454..1348624 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
QU35_RS06890 1348624..1349370 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
QU35_RS06895 1349480..1350481 - 1002 WP_003232642.1 inorganic phosphate transporter -
QU35_RS06900 1350494..1351111 - 618 WP_003218470.1 DUF47 domain-containing protein -
QU35_RS06905 1351387..1352703 - 1317 WP_003244819.1 serine/threonine exchanger -
QU35_RS06910 1353092..1354042 + 951 WP_003245086.1 ring-cleaving dioxygenase -
QU35_RS22385 1354143..1354289 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1314462..1414006 99544


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T50278 WP_003244695.1 NZ_CP010052:c1349370-1348624 [Bacillus subtilis subsp. subtilis str. 168]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T50278 NZ_CP010052:c1349370-1348624 [Bacillus subtilis subsp. subtilis str. 168]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT50278 WP_003232646.1 NZ_CP010052:c1348624-1348454 [Bacillus subtilis subsp. subtilis str. 168]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT50278 NZ_CP010052:c1348624-1348454 [Bacillus subtilis subsp. subtilis str. 168]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References