Detailed information of TA system TA050277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518664..519300
• Replicon: chromosome
• Accession: NZ_CP010052
• Organism: Bacillus subtilis subsp. subtilis str. 168

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: QU35_RS02580
• Protein ID: WP_003156187.1
• Coordinates: 518950..519300 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: QU35_RS02575
• Protein ID: WP_003225183.1
• Coordinates: 518664..518945 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QU35_RS02555	515023..515622	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
QU35_RS02560	515717..516082	+	366	WP_003234281.1	holo-ACP synthase	-
QU35_RS02565	516248..517264	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
QU35_RS02570	517379..518548	+	1170	WP_003234284.1	alanine racemase	-
QU35_RS02575	518664..518945	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
QU35_RS02580	518950..519300	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
QU35_RS02585	519415..520239	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
QU35_RS02590	520244..520609	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
QU35_RS02595	520613..521014	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
QU35_RS02600	521026..522033	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
QU35_RS02605	522095..522424	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
QU35_RS02610	522421..522903	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
QU35_RS02615	522869..523657	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
QU35_RS02620	523657..524256	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T50277 WP_003156187.1 NZ_CP010052:518950-519300 [Bacillus subtilis subsp. subtilis str. 168]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T50277 NZ_CP010052:518950-519300 [Bacillus subtilis subsp. subtilis str. 168]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT50277 WP_003225183.1 NZ_CP010052:518664-518945 [Bacillus subtilis subsp. subtilis str. 168]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT50277 NZ_CP010052:518664-518945 [Bacillus subtilis subsp. subtilis str. 168]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32