Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 20933..21535 Replicon chromosome
Accession NZ_CP010027
Organism Paraburkholderia fungorum strain ATCC BAA-463

Toxin (Protein)


Gene name PumA Uniprot ID A0A2N8Q3W2
Locus tag OI25_RS25235 Protein ID WP_028194450.1
Coordinates 20933..21232 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A0D5VNN5
Locus tag OI25_RS25240 Protein ID WP_028194449.1
Coordinates 21236..21535 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI25_RS25220 17235..18185 - 951 WP_030100587.1 choline ABC transporter substrate-binding protein -
OI25_RS25225 18240..19787 - 1548 WP_046570529.1 choline-sulfatase -
OI25_RS25230 19940..20854 + 915 WP_030100589.1 LysR family transcriptional regulator -
OI25_RS25235 20933..21232 + 300 WP_028194450.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OI25_RS25240 21236..21535 + 300 WP_028194449.1 putative addiction module antidote protein Antitoxin
OI25_RS25245 21707..22582 + 876 WP_030100590.1 formyltetrahydrofolate deformylase -
OI25_RS25250 22686..23543 - 858 WP_028194447.1 glycine betaine ABC transporter substrate-binding protein -
OI25_RS25255 23686..24942 - 1257 WP_096335844.1 hybrid-cluster NAD(P)-dependent oxidoreductase -
OI25_RS25260 24979..26289 - 1311 WP_046570534.1 aromatic ring-hydroxylating dioxygenase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 19940..24942 5002


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-87)

Antitoxin

Cro/C1-type helix-turn-helix domain

(48-92)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11009.69 Da        Isoelectric Point: 9.6247

>T50273 WP_028194450.1 NZ_CP010027:20933-21232 [Paraburkholderia fungorum]
MLKIRTTETFDAWFDGLKDRIARRRIQARIDRLAMGNPGDTNSAGAPIIELRIDHGPGYRVYYVQRGAVLVILLCGGDKS
TQPADIKAAHKMLANLESE

Download         Length: 300 bp

>T50273 NZ_CP010027:20933-21232 [Paraburkholderia fungorum]
ATGTTGAAAATCCGCACCACCGAGACATTTGACGCCTGGTTCGACGGCTTGAAAGACCGGATCGCGAGGCGCCGCATTCA
GGCCCGCATCGACCGTCTGGCGATGGGCAACCCCGGCGACACGAACTCCGCAGGTGCGCCAATTATCGAACTGCGGATCG
ATCACGGTCCCGGCTATCGCGTCTACTACGTGCAGCGCGGCGCAGTGCTGGTGATCCTGCTGTGTGGTGGTGACAAGTCG
ACTCAACCCGCAGATATCAAAGCCGCACACAAGATGCTGGCGAACCTTGAATCGGAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10603.11 Da        Isoelectric Point: 6.0723

>AT50273 WP_028194449.1 NZ_CP010027:21236-21535 [Paraburkholderia fungorum]
MGKIKTRPWDSAEHLKTEADMAEYFEACLVEGGDDPAFLAHALGVIARARGMAQLARDTGISREGLYKALSEDGNPSFAT
IIKVIRALGLELHAAPAHA

Download         Length: 300 bp

>AT50273 NZ_CP010027:21236-21535 [Paraburkholderia fungorum]
ATGGGAAAAATCAAAACCCGGCCATGGGATTCGGCCGAACACCTGAAGACCGAAGCGGACATGGCAGAGTATTTCGAGGC
CTGTCTGGTCGAAGGCGGCGACGATCCTGCATTCCTCGCGCATGCGTTGGGCGTGATCGCGCGTGCGCGCGGCATGGCGC
AACTCGCCCGCGATACCGGGATCTCGCGCGAGGGCTTGTACAAGGCACTCTCCGAAGATGGCAACCCCAGCTTCGCGACG
ATCATCAAGGTGATCCGCGCTCTTGGTCTGGAGTTGCACGCGGCGCCTGCTCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N8Q3W2

Antitoxin

Source ID Structure
AlphaFold DB A0A0D5VNN5

References