Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 266371..266933 Replicon chromosome
Accession NZ_CP010025
Organism Paraburkholderia fungorum strain ATCC BAA-463

Toxin (Protein)


Gene name mazE Uniprot ID I2IBE9
Locus tag OI25_RS03525 Protein ID WP_007183024.1
Coordinates 266371..266691 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID I2IBE8
Locus tag OI25_RS03530 Protein ID WP_007183023.1
Coordinates 266685..266933 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI25_RS03485 261693..262511 + 819 Protein_277 IS21 family transposase -
OI25_RS03490 262498..263265 + 768 WP_046564881.1 IS21-like element helper ATPase IstB -
OI25_RS03495 263262..263480 + 219 WP_046564883.1 hypothetical protein -
OI25_RS03500 263589..264488 - 900 Protein_280 IS110 family transposase -
OI25_RS03505 264595..265131 - 537 Protein_281 IS21 family transposase -
OI25_RS03510 265197..265553 - 357 WP_007183027.1 hypothetical protein -
OI25_RS03515 265575..265910 - 336 WP_007183026.1 hypothetical protein -
OI25_RS03520 266038..266337 - 300 WP_046564889.1 hypothetical protein -
OI25_RS03525 266371..266691 - 321 WP_007183024.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OI25_RS03530 266685..266933 - 249 WP_007183023.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
OI25_RS03535 267117..267578 - 462 WP_082094242.1 SET domain-containing protein-lysine N-methyltransferase -
OI25_RS43080 267682..267969 + 288 WP_158380656.1 hypothetical protein -
OI25_RS03545 268274..269155 - 882 WP_007183022.1 MaoC family dehydratase N-terminal domain-containing protein -
OI25_RS03550 269171..269923 - 753 WP_007183021.1 helix-turn-helix domain-containing protein -
OI25_RS03555 270279..271478 + 1200 WP_007183020.1 acetyl-CoA C-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-104)

Antitoxin

SpoVT-AbrB domain

(9-44)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11898.16 Da        Isoelectric Point: 10.5567

>T50267 WP_007183024.1 NZ_CP010025:c266691-266371 [Paraburkholderia fungorum]
MVMRGEVWLVALDPTLGSEIQKTRPCVVVSPPEMHDHLRTVIVAPMTSKGRPAPFRIPVTFKRKQGLILLDQIRAVDKVR
LVKKEGAVADKTLLDTLRTLQEVFAE

Download         Length: 321 bp

>T50267 NZ_CP010025:c266691-266371 [Paraburkholderia fungorum]
ATGGTAATGCGGGGGGAAGTCTGGTTGGTCGCACTTGATCCGACGCTTGGTAGCGAGATTCAGAAGACGCGCCCCTGTGT
CGTCGTTTCGCCTCCCGAAATGCACGACCACCTGCGCACCGTCATCGTCGCGCCGATGACTTCCAAGGGCCGTCCAGCGC
CGTTTCGGATTCCCGTGACGTTCAAGCGCAAACAGGGTTTGATCCTCCTCGATCAGATTCGCGCAGTAGACAAGGTGCGC
CTGGTCAAGAAAGAGGGCGCAGTTGCCGATAAGACGTTGTTGGATACCCTTCGAACCTTGCAGGAAGTCTTTGCAGAATA
G

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8949.16 Da        Isoelectric Point: 4.6258

>AT50267 WP_007183023.1 NZ_CP010025:c266933-266685 [Paraburkholderia fungorum]
MKTTIRRMGNSQGVLIPKPVLAQLGLESEVEMEVENDAIVLRRPKHKAREGWAEASRALAESGGDALVMGEFSNADDAEL
AW

Download         Length: 249 bp

>AT50267 NZ_CP010025:c266933-266685 [Paraburkholderia fungorum]
ATGAAGACGACGATTCGCAGAATGGGTAATTCGCAGGGCGTGCTGATTCCGAAGCCTGTCCTTGCGCAACTCGGGCTGGA
GAGCGAAGTGGAGATGGAAGTTGAGAACGACGCAATTGTTCTGCGCCGTCCGAAGCACAAAGCCCGTGAGGGTTGGGCGG
AAGCAAGCAGGGCTCTGGCCGAGAGCGGCGGCGACGCGCTGGTCATGGGCGAGTTTTCGAACGCCGATGATGCGGAGCTT
GCATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2T1A0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A2T1A0Q3

References