Detailed information of TA system TA050250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1531988..1532525
• Replicon: chromosome
• Accession: NZ_CP010018
• Organism: Burkholderia thailandensis 34

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: BTM_RS26160
• Protein ID: WP_004549731.1
• Coordinates: 1532256..1532525 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: BTM_RS26155
• Protein ID: WP_045601930.1
• Coordinates: 1531988..1532272 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BTM_RS26130	1528485..1528952	+	468	WP_045598724.1	IS66 family insertion sequence hypothetical protein	-
BTM_RS26135	1528952..1529305	+	354	WP_045598725.1	IS66 family insertion sequence element accessory protein TnpB	-
BTM_RS26140	1529405..1531027	+	1623	WP_045598726.1	IS66 family transposase	-
BTM_RS26145	1531037..1531297	-	261	WP_045598729.1	hypothetical protein	-
BTM_RS26150	1531387..1531584	-	198	WP_045601929.1	hypothetical protein	-
BTM_RS26155	1531988..1532272	-	285	WP_045601930.1	BrnA antitoxin family protein	Antitoxin
BTM_RS26160	1532256..1532525	-	270	WP_004549731.1	BrnT family toxin	Toxin
BTM_RS26165	1532575..1533018	-	444	WP_045601932.1	hypothetical protein	-
BTM_RS35370	1533301..1534058	+	758	WP_186288326.1	IS5 family transposase	-
BTM_RS26185	1534086..1535284	+	1199	WP_102889818.1	IS3 family transposase	-
BTM_RS26190	1535688..1536422	-	735	WP_009893914.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1515410..1535284	19874
	-	inside	IScluster/Tn	-	-	1527589..1535284	7695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T50250 WP_004549731.1 NZ_CP010018:c1532525-1532256 [Burkholderia thailandensis 34]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T50250 NZ_CP010018:c1532525-1532256 [Burkholderia thailandensis 34]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCACGGGGTGTCGTTGGCGCTTGCGGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10655.06 Da
• Isoelectric Point: 5.8930

>AT50250 WP_045601930.1 NZ_CP010018:c1532272-1531988 [Burkholderia thailandensis 34]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDVVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT50250 NZ_CP010018:c1532272-1531988 [Burkholderia thailandensis 34]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGTCGAGGTAAGCGCGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGATGTCGTCGACGCCTTCAAGGCGACGGGCGAAGGTTGGCAGACGCGGATG
AACGACGCGCTGCGCGAGTGGTTGAAGGAGCATCAACCGGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2