Detailed information of TA system TA050239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4040326..4040944
• Replicon: chromosome
• Accession: NZ_CP009997
• Organism: Yersinia kristensenii strain Y231

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: CH54_RS18220
• Protein ID: WP_002208622.1
• Coordinates: 4040326..4040529 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A386HG68
• Locus tag: CH54_RS18225
• Protein ID: WP_038631539.1
• Coordinates: 4040576..4040944 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH54_RS18195	4035623..4035961	+	339	WP_002208627.1	P-II family nitrogen regulator	-
CH54_RS18200	4036001..4037290	+	1290	WP_038639390.1	ammonium transporter AmtB	-
CH54_RS18205	4037372..4038232	-	861	WP_038631531.1	acyl-CoA thioesterase II	-
CH54_RS18210	4038490..4039014	+	525	WP_038631533.1	YbaY family lipoprotein	-
CH54_RS18215	4039058..4039369	-	312	WP_186368485.1	MGMT family protein	-
CH54_RS18220	4040326..4040529	-	204	WP_002208622.1	expression modulating protein YmoA	Toxin
CH54_RS18225	4040576..4040944	-	369	WP_038631539.1	Hha toxicity modulator TomB	Antitoxin
CH54_RS18230	4041103..4041456	-	354	WP_038631541.1	hypothetical protein	-
CH54_RS18235	4042223..4042951	+	729	WP_038631543.1	ABC transporter ATP-binding protein	-
CH54_RS18240	4042948..4043805	+	858	WP_038631544.1	metal ABC transporter permease	-
CH54_RS18245	4043852..4044730	+	879	WP_038631545.1	metal ABC transporter substrate-binding protein	-
CH54_RS18250	4044852..4044995	-	144	WP_038631546.1	type B 50S ribosomal protein L36	-
CH54_RS18255	4045011..4045271	-	261	WP_038631547.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T50239 WP_002208622.1 NZ_CP009997:c4040529-4040326 [Yersinia kristensenii]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T50239 NZ_CP009997:c4040529-4040326 [Yersinia kristensenii]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACAACTATTGATACATTAGAACGTGTAATTGAAAAGAATAA
GTACGAACTTTCAGATGATGAGCTGGAATTATTTTATTCAGCTGCAGACCATCGCTTAGCTGAGCTCACAATGAATAAAC
TTTATGATAAAATTCCACCTACTGTGTGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14190.97 Da
• Isoelectric Point: 4.5140

>AT50239 WP_038631539.1 NZ_CP009997:c4040944-4040576 [Yersinia kristensenii]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDGADLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYICTLMKT

Nucleotide

• Length: 369 bp

>AT50239 NZ_CP009997:c4040944-4040576 [Yersinia kristensenii]
ATGGATGAGTACTCACCGAAGCGGCATGACATTGCCCAATTGAAGTTTTTATGTGAGAGTTTGTATGATGAAGGCATTGC
CACCCTTGGTGACAGCCACCATGGTTGGGTAAATGACCCGACTTCTGCCGTAAATCTACAATTGAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCTGATGGCGCAGACCTTTCAGAGTTAGTGGAAGAATATTTA
GACGATACATATACTCTGTTTAGTAGTTATGGGATCAATGACCCTGAATTGCGTCGCTGGCAAAAAACCAAAGAGCGTTT
ATTCAGACTTTTCTCAGGGGAATACATCTGTACCCTGATGAAAACTTAA

Structures

Toxin

• PDB: 2K5S