Detailed information of TA system TA050236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 2319601..2320264
• Replicon: chromosome
• Accession: NZ_CP009997
• Organism: Yersinia kristensenii strain Y231

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A0T9UMV8
• Locus tag: CH54_RS10460
• Protein ID: WP_004705513.1
• Coordinates: 2319601..2319945 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: CH54_RS10465
• Protein ID: WP_004705512.1
• Coordinates: 2319947..2320264 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH54_RS10440	2315896..2317134	-	1239	Protein_2044	IS110 family transposase	-
CH54_RS10445	2317908..2318156	+	249	WP_042562560.1	hypothetical protein	-
CH54_RS10450	2318179..2318823	+	645	WP_042562559.1	hypothetical protein	-
CH54_RS10455	2319059..2319241	+	183	WP_042562558.1	hypothetical protein	-
CH54_RS10460	2319601..2319945	+	345	WP_004705513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH54_RS10465	2319947..2320264	+	318	WP_004705512.1	helix-turn-helix domain-containing protein	Antitoxin
CH54_RS10470	2320389..2320742	-	354	WP_042562557.1	helix-turn-helix domain-containing protein	-
CH54_RS10480	2321144..2321476	+	333	WP_042562555.1	hypothetical protein	-
CH54_RS10485	2321654..2324452	-	2799	WP_080868721.1	conjugative transfer ATPase	-
CH54_RS10490	2324452..2324862	-	411	WP_042562554.1	TIGR03751 family conjugal transfer lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2281206..2353034	71828

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12448.25 Da
• Isoelectric Point: 9.7663

>T50236 WP_004705513.1 NZ_CP009997:2319601-2319945 [Yersinia kristensenii]
MKPLYWVGSSKKDLQSLPEDVQDIFGYGLHLAQMGSKHSQAKPLKGFGGAGVLEVVEDYIGDTYRAVYTVKFGHAVYVLH
AFQKKSSSGIATPKPDVDKIRERLKAAESHAKGA

Nucleotide

• Length: 345 bp

>T50236 NZ_CP009997:2319601-2319945 [Yersinia kristensenii]
ATGAAGCCACTTTATTGGGTCGGTAGCAGTAAGAAGGATCTCCAATCTCTACCTGAAGATGTACAAGATATTTTCGGCTA
CGGCCTGCATTTGGCACAGATGGGCAGTAAACATTCCCAAGCTAAGCCATTGAAAGGGTTTGGTGGTGCGGGTGTACTGG
AAGTTGTTGAGGATTATATCGGTGATACCTACCGAGCGGTTTACACTGTTAAATTTGGTCATGCGGTGTATGTGTTACAT
GCATTCCAGAAAAAATCTTCATCAGGAATAGCCACGCCAAAGCCAGATGTGGATAAGATCCGCGAGCGGCTGAAAGCCGC
AGAAAGTCATGCTAAAGGAGCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11529.44 Da
• Isoelectric Point: 10.6107

>AT50236 WP_004705512.1 NZ_CP009997:2319947-2320264 [Yersinia kristensenii]
MSHDIEISSGNVYADLGKDNAEEMQVKAQLATTIGNIIKSRRLTQEQAAKLLGMTQPKLSNMLRGQFRGISEAKMLECLT
RLGRDVQIVVGKARRTPGSLKVVFA

Nucleotide

• Length: 318 bp

>AT50236 NZ_CP009997:2319947-2320264 [Yersinia kristensenii]
ATGAGCCATGATATCGAAATCAGCAGCGGTAACGTTTATGCCGATCTTGGCAAAGATAATGCTGAAGAAATGCAGGTAAA
AGCACAGCTTGCTACCACTATTGGTAACATTATTAAAAGCCGCCGCCTGACACAAGAACAGGCCGCCAAGCTGCTGGGGA
TGACTCAGCCTAAGCTGTCTAATATGTTGCGCGGGCAATTTCGAGGCATTAGCGAGGCCAAAATGTTGGAATGCCTGACC
CGTTTGGGGCGAGATGTGCAAATTGTCGTCGGGAAAGCGCGCCGCACTCCTGGTAGCCTGAAAGTGGTGTTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0T9UMV8