Detailed information of TA system TA050209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Phd(antitoxin)
• Location: 1512621..1513168
• Replicon: chromosome
• Accession: NZ_CP009984
• Organism: Vibrio vulnificus strain FORC_009

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2K7SRN5
• Locus tag: FORC9_RS07040
• Protein ID: WP_010448451.1
• Coordinates: 1512866..1513168 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U3A269
• Locus tag: FORC9_RS07035
• Protein ID: WP_005448240.1
• Coordinates: 1512621..1512878 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC9_RS07000	1507743..1508348	+	606	WP_060531664.1	hypothetical protein	-
FORC9_RS23540	1508820..1508969	+	150	WP_081036233.1	DUF3265 domain-containing protein	-
FORC9_RS07005	1508966..1509637	+	672	WP_081036234.1	HAD-IA family hydrolase	-
FORC9_RS24195	1510135..1510302	-	168	Protein_1489	IS30 family transposase	-
FORC9_RS23545	1511739..1511831	+	93	WP_080772459.1	DUF3265 domain-containing protein	-
FORC9_RS07025	1511893..1512192	-	300	WP_060531668.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FORC9_RS07030	1512200..1512442	-	243	WP_039553847.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FORC9_RS07035	1512621..1512878	+	258	WP_005448240.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FORC9_RS07040	1512866..1513168	+	303	WP_010448451.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC9_RS07045	1513339..1513803	+	465	WP_060531669.1	ASCH domain-containing protein	-
FORC9_RS07050	1513837..1514805	-	969	WP_081036190.1	IS30 family transposase	-
FORC9_RS07055	1514928..1515884	-	957	WP_060531432.1	IS110 family transposase	-
FORC9_RS24210	1516119..1516178	+	60	Protein_1499	DUF3265 domain-containing protein	-
FORC9_RS23555	1516179..1516576	-	398	Protein_1500	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1346398..1521281	174883
	-	inside	IScluster/Tn	-	-	1505354..1515884	10530
	-	inside	Integron	-	-	1472484..1513803	41319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11625.52 Da
• Isoelectric Point: 5.1765

>T50209 WP_010448451.1 NZ_CP009984:1512866-1513168 [Vibrio vulnificus]
MAEVIWTEPALSDLNDIAEYIALENIVAAKQLVQTIFSKVERLQTFPESGRIPPELEHLSYREVVVNPCRVFYKQDGDKV
FILFVMRAERDLRKFLLGKQ

Nucleotide

• Length: 303 bp

>T50209 NZ_CP009984:1512866-1513168 [Vibrio vulnificus]
ATGGCTGAAGTAATCTGGACTGAACCAGCGTTATCTGACCTCAATGATATCGCTGAATACATCGCACTTGAAAATATCGT
AGCTGCAAAGCAGTTAGTACAAACGATCTTCTCCAAAGTCGAACGCCTACAAACTTTCCCAGAGTCAGGTCGTATTCCAC
CCGAGCTAGAACATTTAAGTTATCGTGAAGTTGTCGTTAATCCATGTCGTGTTTTCTATAAACAAGACGGTGACAAAGTG
TTTATTCTGTTTGTTATGCGTGCAGAGAGAGATTTGCGTAAGTTCTTGTTGGGTAAGCAATAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9606.10 Da
• Isoelectric Point: 6.7269

>AT50209 WP_005448240.1 NZ_CP009984:1512621-1512878 [Vibrio vulnificus]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLVDVDDYEFMQNRLAILEGIARGERALADGKVVSHDEAKDKM
SKWLK

Nucleotide

• Length: 258 bp

>AT50209 NZ_CP009984:1512621-1512878 [Vibrio vulnificus]
ATGAAAGTAGAACTAGTTACATCACTCAAACGTCAAGCAACTAAGATCCTCGCCGATCTCCACGACACCAAAGAACCAGT
GTTAATTACTGAGCATGGTAAGCCATCTGCGTATCTAGTTGATGTTGATGACTACGAATTTATGCAAAATCGTCTAGCTA
TTCTTGAGGGTATTGCACGAGGTGAGCGTGCACTAGCTGATGGTAAAGTTGTAAGTCATGATGAAGCCAAGGACAAAATG
TCAAAATGGCTGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2K7SRN5

Antitoxin

• AlphaFold DB: A0A432DGX8