Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4249988..4250630 Replicon chromosome
Accession NZ_CP009981
Organism Bacillus anthracis strain Ames isolate BACI008

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BF25_RS23995 Protein ID WP_000635963.1
Coordinates 4249988..4250338 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BF25_RS24000 Protein ID WP_000004570.1
Coordinates 4250343..4250630 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BF25_RS23980 4246924..4247382 - 459 WP_000344248.1 SprT family protein -
BF25_RS23985 4247578..4247694 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BF25_RS23990 4247752..4249920 - 2169 WP_000426223.1 RNA-binding transcriptional accessory protein -
BF25_RS23995 4249988..4250338 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BF25_RS24000 4250343..4250630 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BF25_RS24005 4250940..4252109 - 1170 WP_000390596.1 alanine racemase -
BF25_RS24010 4252228..4253178 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BF25_RS24015 4253335..4253694 - 360 WP_000635040.1 holo-ACP synthase -
BF25_RS24020 4253787..4254359 + 573 WP_000906916.1 rhomboid family intramembrane serine protease -
BF25_RS24025 4254352..4255314 - 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T50201 WP_000635963.1 NZ_CP009981:c4250338-4249988 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T50201 NZ_CP009981:c4250338-4249988 [Bacillus anthracis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGTGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT50201 WP_000004570.1 NZ_CP009981:c4250630-4250343 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT50201 NZ_CP009981:c4250630-4250343 [Bacillus anthracis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References