Detailed information of TA system TA050200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2174088..2175063
• Replicon: chromosome
• Accession: NZ_CP009981
• Organism: Bacillus anthracis strain Ames isolate BACI008

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A2R2IKP2
• Locus tag: BF25_RS13010
• Protein ID: WP_002036394.1
• Coordinates: 2174326..2175063 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: BF25_RS13005
• Protein ID: WP_000588712.1
• Coordinates: 2174088..2174213 (-)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BF25_RS12975	2169842..2170312	+	471	WP_000670597.1	DUF5065 family protein	-
BF25_RS12980	2170876..2172345	-	1470	WP_000287522.1	beta-Ala-His dipeptidase	-
BF25_RS31840	2172489..2172665	-	177	WP_000808045.1	hypothetical protein	-
BF25_RS12990	2173004..2173240	+	237	WP_001021689.1	hypothetical protein	-
BF25_RS12995	2173517..2173798	+	282	WP_001041555.1	hypothetical protein	-
BF25_RS13000	2173835..2174011	-	177	WP_000852614.1	hypothetical protein	-
BF25_RS13005	2174088..2174213	-	126	WP_000588712.1	hypothetical protein	Antitoxin
BF25_RS13010	2174326..2175063	-	738	WP_002036394.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BF25_RS13015	2175252..2175989	-	738	WP_000594139.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BF25_RS13020	2176157..2176639	-	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
BF25_RS13025	2176746..2178431	+	1686	WP_009879073.1	alpha-keto acid decarboxylase family protein	-
BF25_RS13030	2178553..2179335	-	783	WP_000381787.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.03 Da
• Isoelectric Point: 8.2705

>T50200 WP_002036394.1 NZ_CP009981:c2175063-2174326 [Bacillus anthracis]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T50200 NZ_CP009981:c2175063-2174326 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT50200 WP_000588712.1 NZ_CP009981:c2174213-2174088 [Bacillus anthracis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT50200 NZ_CP009981:c2174213-2174088 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: A0A2R2IKP2

Antitoxin

• AlphaFold DB: A0A366GQT1