Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 4355027..4355910 Replicon chromosome
Accession NZ_CP009974
Organism Pseudomonas putida S12

Toxin (Protein)


Gene name - Uniprot ID A0A2K4LSC9
Locus tag RPPX_RS19380 Protein ID WP_012052908.1
Coordinates 4355473..4355910 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A524BX56
Locus tag RPPX_RS19375 Protein ID WP_014591577.1
Coordinates 4355027..4355476 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RPPX_RS19350 4350452..4351651 + 1200 WP_014591581.1 sugar transporter -
RPPX_RS19355 4351691..4352275 - 585 WP_014591580.1 LysE family transporter -
RPPX_RS19360 4352326..4353156 - 831 WP_003250518.1 AraC family transcriptional regulator -
RPPX_RS19365 4353205..4354128 - 924 WP_019438774.1 LysR family transcriptional regulator -
RPPX_RS19370 4354232..4354885 + 654 WP_014591578.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
RPPX_RS19375 4355027..4355476 + 450 WP_014591577.1 DUF2384 domain-containing protein Antitoxin
RPPX_RS19380 4355473..4355910 + 438 WP_012052908.1 RES family NAD+ phosphorylase Toxin
RPPX_RS19385 4355968..4357122 - 1155 WP_019438775.1 aminotransferase class V-fold PLP-dependent enzyme -
RPPX_RS19390 4357316..4358242 - 927 WP_014591574.1 LysR family transcriptional regulator -
RPPX_RS19395 4358383..4359612 + 1230 WP_003250533.1 acyl-CoA dehydrogenase -
RPPX_RS19400 4359708..4360772 - 1065 WP_014591573.1 TerC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(97-146)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15944.21 Da        Isoelectric Point: 5.6016

>T50198 WP_012052908.1 NZ_CP009974:4355473-4355910 [Pseudomonas putida S12]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDGSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Download         Length: 438 bp

>T50198 NZ_CP009974:4355473-4355910 [Pseudomonas putida S12]
GTGATTTTGTGGCGAATCAGCGCCTATGCAGACTTGAGCGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGTCGGCCAGTGGTGTATGCCGCCACCAGCCCTCCCGGGGCGATGCTGGAAGTGTTGGTGCACCTGGAGATCGACCCCG
AAGATTTCCCTACCACCATGCGCTTGCTGCGCATCGAACTGCCCGACACGGTTTCCCAGGCGCAGTTGCCCGCTTTGCAG
CCCGGCTGGTCTGCCCAACCTGAGTTGACCCGTACGCTGGGCAATCGCTTTCTGGATGACGGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACCCCGGACAGCCGCCTGTTCTAG

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16984.51 Da        Isoelectric Point: 5.7258

>AT50198 WP_014591577.1 NZ_CP009974:4355027-4355476 [Pseudomonas putida S12]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEVGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Download         Length: 450 bp

>AT50198 NZ_CP009974:4355027-4355476 [Pseudomonas putida S12]
ATGCTTGCCGAAGTGCTACGCGACAACGGTTACCACGAGTACCGGGCGCGCTTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGTGACTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACTGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATTCCGCTGCGCACCCTCAAATCCCGTATCGAGCGTGACCAACCGCTGACC
GTGGATGAGAGCGACCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAGGCTGTGTTCGGGGAAGTGGGCAAGGC
CAAACGCTGGCTTTCCAAGCCTAAGGAACGCTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4LSC9

Antitoxin

Source ID Structure
AlphaFold DB A0A524BX56

References