50177

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5140924..5141566 Replicon chromosome
Accession NZ_CP009941
Organism Bacillus cereus strain 03BB87

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag NT98_RS27670 Protein ID WP_000635963.1
Coordinates 5140924..5141274 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag NT98_RS27675 Protein ID WP_000004570.1
Coordinates 5141279..5141566 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NT98_RS27655 5137860..5138318 - 459 WP_001975527.1 SprT family protein -
NT98_RS27660 5138514..5138630 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
NT98_RS27665 5138688..5140856 - 2169 WP_001975517.1 RNA-binding transcriptional accessory protein -
NT98_RS27670 5140924..5141274 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NT98_RS27675 5141279..5141566 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
NT98_RS27680 5141876..5143045 - 1170 WP_000390607.1 alanine racemase -
NT98_RS27685 5143163..5144113 - 951 WP_033691678.1 outer membrane lipoprotein carrier protein LolA -
NT98_RS27690 5144270..5144629 - 360 WP_000635047.1 holo-ACP synthase -
NT98_RS27695 5144722..5145294 + 573 WP_001975497.1 rhomboid family intramembrane serine protease -
NT98_RS27700 5145287..5146249 - 963 WP_000961159.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T50177 WP_000635963.1 NZ_CP009941:c5141274-5140924 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T50177 NZ_CP009941:c5141274-5140924 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT50177 WP_000004570.1 NZ_CP009941:c5141566-5141279 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT50177 NZ_CP009941:c5141566-5141279 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References