Detailed information of TA system TA050176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3021156..3022131
• Replicon: chromosome
• Accession: NZ_CP009941
• Organism: Bacillus cereus strain 03BB87

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: NT98_RS16890
• Protein ID: WP_033691453.1
• Coordinates: 3021394..3022131 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: NT98_RS16885
• Protein ID: WP_040119960.1
• Coordinates: 3021156..3021281 (-)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NT98_RS16860	3016173..3016790	-	618	WP_142244172.1	SGNH/GDSL hydrolase family protein	-
NT98_RS16865	3017123..3017782	-	660	WP_001979058.1	CatB-related O-acetyltransferase	-
NT98_RS16870	3018237..3018695	+	459	Protein_3060	DUF5065 family protein	-
NT98_RS16875	3019286..3020760	-	1475	Protein_3061	beta-Ala-His dipeptidase	-
NT98_RS31825	3020904..3021080	-	177	WP_040119959.1	hypothetical protein	-
NT98_RS16885	3021156..3021281	-	126	WP_040119960.1	hypothetical protein	Antitoxin
NT98_RS16890	3021394..3022131	-	738	WP_033691453.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
NT98_RS16895	3022320..3023057	-	738	WP_001980023.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
NT98_RS16900	3023224..3023706	-	483	WP_000191902.1	MarR family winged helix-turn-helix transcriptional regulator	-
NT98_RS16905	3023813..3025496	+	1684	Protein_3067	alpha-keto acid decarboxylase family protein	-
NT98_RS16910	3025673..3026455	-	783	WP_001980020.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28433.19 Da
• Isoelectric Point: 8.2672

>T50176 WP_033691453.1 NZ_CP009941:c3022131-3021394 [Bacillus cereus]
VISNIRIGLFILVIVFLVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEVAKELNTNFRGIRSQFSTMIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T50176 NZ_CP009941:c3022131-3021394 [Bacillus cereus]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGTAATCGTTTTTCTAGTACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCGAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACAGTTCAATCGATAAAACGTTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGTGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACTATGATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4846.21 Da
• Isoelectric Point: 3.9507

>AT50176 WP_040119960.1 NZ_CP009941:c3021281-3021156 [Bacillus cereus]
VIKNNDNDFFKEDNEDTTDFSLIKGDTVQQVEDLEDELKRK

Nucleotide

• Length: 126 bp

>AT50176 NZ_CP009941:c3021281-3021156 [Bacillus cereus]
GTGATAAAAAATAATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACGGATTTCTCGTTAATTAAAGGGGATAC
GGTGCAGCAAGTAGAAGATTTAGAAGATGAATTAAAAAGAAAATGA