Detailed information of TA system TA050175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1839917..1840834
• Replicon: chromosome
• Accession: NZ_CP009938
• Organism: Bacillus sp. BH072 isolate honey

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: OY17_RS09440
• Protein ID: WP_007407256.1
• Coordinates: 1840088..1840834 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: OY17_RS09435
• Protein ID: WP_014417527.1
• Coordinates: 1839917..1840087 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OY17_RS09390	1835123..1835635	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
OY17_RS20460	1835748..1836407	+	660	WP_014417523.1	terminase	-
OY17_RS09400	1836305..1836772	+	468	Protein_1812	hypothetical protein	-
OY17_RS09405	1836785..1837156	+	372	WP_014417525.1	hypothetical protein	-
OY17_RS09410	1837161..1837358	+	198	WP_007610833.1	XkdX family protein	-
OY17_RS09415	1837415..1838176	+	762	WP_014417526.1	hypothetical protein	-
OY17_RS09420	1838228..1838491	+	264	WP_003154815.1	hemolysin XhlA family protein	-
OY17_RS09425	1838505..1838768	+	264	WP_003154813.1	phage holin	-
OY17_RS09430	1838782..1839660	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
OY17_RS09435	1839917..1840087	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
OY17_RS09440	1840088..1840834	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
OY17_RS09445	1840938..1841936	-	999	WP_003154805.1	inorganic phosphate transporter	-
OY17_RS09450	1841949..1842566	-	618	WP_003154804.1	DUF47 domain-containing protein	-
OY17_RS09455	1842852..1844168	-	1317	WP_007610842.1	amino acid permease	-
OY17_RS09460	1844490..1845440	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T50175 WP_007407256.1 NZ_CP009938:c1840834-1840088 [Bacillus sp. BH072]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T50175 NZ_CP009938:c1840834-1840088 [Bacillus sp. BH072]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT50175 WP_014417527.1 NZ_CP009938:c1840087-1839917 [Bacillus sp. BH072]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT50175 NZ_CP009938:c1840087-1839917 [Bacillus sp. BH072]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8