Detailed information of TA system TA050136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 39263..39797
• Replicon: plasmid pCD
• Accession: NZ_CP009905
• Organism: Yersinia pestis Antiqua

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: CH58_RS00870
• Protein ID: WP_002213360.1
• Coordinates: 39263..39562 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: CH58_RS00875
• Protein ID: WP_002224342.1
• Coordinates: 39555..39797 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CH58_RS00835	34697..35122	-	426	WP_002213267.1	type III secretion system chaperone SycH	-
CH58_RS00840	35498..35896	-	399	Protein_46	ATP-binding protein	-
CH58_RS00845	35896..37068	-	1173	WP_002220893.1	IS21 family transposase	-
CH58_RS00855	37171..37815	+	645	Protein_48	IS3 family transposase	-
CH58_RS00860	37878..38270	-	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
CH58_RS00865	38464..39123	+	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
CH58_RS00870	39263..39562	-	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CH58_RS00875	39555..39797	-	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
CH58_RS24895	39963..40255	-	293	Protein_53	IS6 family transposase	-
CH58_RS26065	40340..40513	+	174	WP_002213357.1	hypothetical protein	-
CH58_RS25875	40662..40898	-	237	WP_154020274.1	hypothetical protein	-
CH58_RS00885	41058..42023	-	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
CH58_RS00890	42020..43186	-	1167	WP_002220902.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virG/yscW / exsA / yscB / yscC / yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / yopH / yopJ/yopP / yopO/ypkA / yadA / yadA / sycH / sycE/yerA / yopE / yopK/yopQ / yopT / sycT / yopM / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN / yscN / yscO / yscP / yscQ / yscR / yscS / yscT / yscU	1..70290	70290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T50136 WP_002213360.1 NZ_CP009905:c39562-39263 [Yersinia pestis Antiqua]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T50136 NZ_CP009905:c39562-39263 [Yersinia pestis Antiqua]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT50136 WP_002224342.1 NZ_CP009905:c39797-39555 [Yersinia pestis Antiqua]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT50136 NZ_CP009905:c39797-39555 [Yersinia pestis Antiqua]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6