Detailed information of TA system TA050128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 43734..44404
• Replicon: plasmid pXF64-HB
• Accession: NZ_CP009886
• Organism: Xylella fastidiosa strain Hib4

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: XFHB_RS13990
• Protein ID: WP_088578693.1
• Coordinates: 43734..44084 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: XFHB_RS13995
• Protein ID: WP_088578668.1
• Coordinates: 44081..44404 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFHB_RS13955	39125..39433	-	309	WP_088578663.1	hypothetical protein	-
XFHB_RS13960	39592..40311	-	720	WP_088578664.1	DUF3560 domain-containing protein	-
XFHB_RS13970	41051..41329	-	279	WP_088578665.1	transcriptional regulator	-
XFHB_RS13980	41480..42685	-	1206	WP_088578667.1	ParB/RepB/Spo0J family partition protein	-
XFHB_RS13985	42682..43446	-	765	WP_010895252.1	ParA family protein	-
XFHB_RS13990	43734..44084	+	351	WP_088578693.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
XFHB_RS13995	44081..44404	+	324	WP_088578668.1	helix-turn-helix domain-containing protein	Antitoxin
XFHB_RS14000	44747..45013	-	267	WP_088578669.1	hypothetical protein	-
XFHB_RS14005	45010..45645	-	636	WP_088578670.1	AAA family ATPase	-
XFHB_RS14010	46489..47328	-	840	WP_088578694.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64251	64251
	-	flank	IS/Tn	-	-	38614..39192	578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-82)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12782.70 Da
• Isoelectric Point: 8.0810

>T50128 WP_088578693.1 NZ_CP009886:43734-44084 [Xylella fastidiosa]
MKPLVWIASAKKDLMAMPDDVQDTFGYALHLAQIGEKHDQAKPLKGFGSASVLEVVEDSDGGTYRGVYTVRFGNAVYVLH
CFQKKSTHGIATPKPDIDLIRDRLKVAETHAKGELK

Nucleotide

• Length: 351 bp

>T50128 NZ_CP009886:43734-44084 [Xylella fastidiosa]
ATGAAACCGCTAGTTTGGATCGCGTCGGCCAAGAAAGACCTGATGGCGATGCCGGACGACGTACAGGACACTTTCGGGTA
TGCGCTCCATTTGGCTCAGATCGGCGAGAAGCACGACCAGGCCAAGCCGCTCAAAGGATTCGGTTCGGCTAGCGTGCTCG
AAGTGGTCGAGGATTCAGACGGCGGCACATACCGCGGGGTCTACACGGTGCGATTCGGAAACGCGGTGTATGTCCTGCAT
TGCTTCCAGAAGAAATCGACGCATGGCATAGCGACACCAAAGCCGGATATCGACCTCATTCGAGATCGATTGAAGGTCGC
CGAGACGCACGCGAAAGGAGAACTGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11491.39 Da
• Isoelectric Point: 6.9806

>AT50128 WP_088578668.1 NZ_CP009886:44081-44404 [Xylella fastidiosa]
MIEIEEGSGNVYADLGIPDAGEMLVKAQLATKIGEIIKGRGWTQQEAANVLGMTQPKLSKMLRGQFRGISEAKMLECLAR
LGRQVQIVVGPARLTNDAGHVEVVFAA

Nucleotide

• Length: 324 bp

>AT50128 NZ_CP009886:44081-44404 [Xylella fastidiosa]
ATGATCGAAATTGAAGAAGGTAGCGGCAACGTCTATGCCGATCTTGGCATTCCTGACGCCGGCGAAATGCTGGTGAAGGC
GCAGCTTGCTACGAAGATCGGCGAGATCATCAAAGGTCGCGGATGGACTCAGCAAGAAGCGGCCAACGTGCTCGGCATGA
CACAACCCAAGCTGTCGAAGATGCTGCGCGGACAGTTCCGAGGTATCAGCGAAGCGAAGATGCTGGAATGCCTCGCGCGG
CTCGGTCGGCAAGTTCAGATCGTCGTCGGTCCCGCTCGCCTTACAAACGACGCGGGCCATGTTGAAGTCGTATTCGCAGC
CTAA